We narrowed to 798 results for: Arc
-
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....conference. It was inspiring to see how microbiology research is improving lives worldwide and the presentation...the booth. We were happy to chat with lots of researchers who weren’t aware of Addgene’s plasmid sharing... plasmid tools on the horizon for microbiology research and we’d love to help more microbiologists share...being the Director of the Great Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim...
-
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....tagging method that labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6...Guo, Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics...generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in model organisms... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent...rather than cyan and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation...function of the protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both...posts about choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....visibly complex - and unique - adult. My initial research directions followed that avenue with projects ...interests and skills than what fits for applied research. I focus in general terms on a couple of very ...teaching – often explaining a technical piece of research to others outside that specialty, who may be other...professional meetings, societies, networks just as do research scientists. There we meet others in the scientific... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....also bring benefits to researchers conducting basic or early pre-clinical research. However, standard terms...concept of gRNA design is still challenging for researchers using CRISPR, and that standardizing and improving...resources and publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....genome and proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful...replication When it comes to replication, viruses tend to march to the beat of their own drum. They have a wide ... eliminate or reduce the risk of infection — researchers were able to create far safer viral vectors that... The four types of viral vectors (which many researchers will informally refer to as viruses) are named... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....H134R)-EYFP Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns...sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters... viral protocols and videos Find AAVs for your research ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively...believe that these results will be beneficial to the research community. So, why are we so excited? For starters...inventory and more time providing new materials to the research community. References 1. Coroadinha, A. S., ... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....Although plasmids started as a somewhat niche area of research, they are now seen as an ubiquitous tool that ...little bit easier for scientists to conduct their research! Since their discovery in the 1950s, plasmids ...have greatly impacted many areas of biological research and have been key in advancing our knowledge in... Marcy Patrick... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....downward trend in funding and space in academic research labs has more students looking for other options...perspectives in science and engineering Looking at your research from a broader viewpoint may expose which experiments...extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking a break... skill sets Taking the time to identify and research 2 – 3 potential professions will highlight which... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....increasingly more cost effective and precise, researchers are now using it to investigate potential therapeutics...Alzhiemer’s disease (AD). Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...correct disease hadn’t yet been realized. The researchers chose to explore its potential in mice with a...known as Crygc. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found that affect... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on educational...students in functional genomics research Learn how the Arabidopsis research center brings plant science to... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...lentiviral prep here Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns... viral protocols and videos Find AAVs for your research ... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...10.3791/54832 Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies...https://doi.org/10.1074/jbc.m116.736942 National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m.... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...overexpression of all human transcription factors, barcoded libraries to trace cell lineage, and so much more... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....method. Read on to learn how to use it in your research! The starting point: Ligation-independent cloning...only one type of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m.... Optogenetics Plasmids for Use in Neuroscience Research Neuroscience covers many subfields When I first...neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect Alzheimer's...projects using biological tools. In addition to the researcher using sequencing technologies to study microbiome...making more AAVs and educational content to help researchers use these great tools. If you’re just getting...