We narrowed to 364 results for: DUR
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TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than...
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PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....mutations seen with TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....there are many factors that need to be considered during the planning process. For CRISPR experiments, the... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable, easily purified protein... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....various mammalian promoters), the same cloning procedure can be used to incorporate each element into the... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....al., 2006. Tips: Don’t plate seeds too densely during selection: this will increase background growth... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m..... For transposons to be specifically recognized during transposition, genetic cargos must be flanked by... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....aggregation and methods to prevent its occurrence during vector purification and formulation. Molecular ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully expressed... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....tubes help to guarantee that no samples get lost during their journey. Take the time to track your samples... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....activity – it only requires the presence of oxygen during maturation. This meant that the protein could be... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....lab, low copy plasmids may result in low yields during DNA purification. On the other hand, they also ... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m.... of the department] at [name of the university].During my thesis work on [phenomenon X], I read several... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....skewed the representation of your pooled library during the amplification process. It’s far better to verify... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....overlooked. Nanobodies are smaller in size, more durable, and usually much easier to clone, express, and... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m....Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia...