We narrowed to 700 results for: EED

...mechanisms and, eventually, develop treatments, we need tools to specifically edit methylation sites.  Step... DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise it will continue to ...nuclear environment. One good system to balance these needs is the lentiviral-based expression of floxed dCas9...

...produced in animals may be variable among animals and bleed dates and even clonal hybridoma cell lines can produce... Made? To make recombinant antibodies, you first need to know the sequence. Scientists are getting better...friends! Finally, if we want better antibodies, we need to empower the scientific community with the tools...

...number, media volume, and plasmid concentrations will need to be optimized prior to starting. We recommend ... experimental volumes accordingly. While you may need to make some final adjustments, this is generally... to start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt...

... And finally, provide feedback. If you are unsatisfied with the nature or speed of a response, let your...know (nicely, please!). We can’t improve without feedback. And if you had a very positive experience, also...

...switch can be created for your specifc experimental needs. How does the FLEx switch work? Let’s say you want... switch to successfully work, the cassette would need to contain the BFP coding sequence in the sense ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

...signal input to readable output, which generally exceeds that of the VSD GEVIs. However, since rhodopsin... specific targeting of these voltage indicators needs to be cell type specific as well as specifically...10.1186/s12915-019-0682-0. Beck, C., Gong, Y. A high-speed, bright, red fluorescent voltage sensor to detect...

...note, there is no minimum number of markers that needs to be used with spectral flow—you can run a three...co-expression among cellular markers You’ll also need to know if markers are co-expressed in your cells...have a level of background autofluorescence that needs to be accounted for in order to distinguish the ...

...marker proteins, however, in this case the cell needs to be fixed and the cell membrane permeabilized ...problems associated with fluorescent imaging such as bleed-through (the incorrect detection of one fluorescent...baseline rule, the emission spectra of the selected FPs need to be sufficiently separated, most commonly FPs ...

...planning, you’ll know exactly what materials you’ll need to bring into the hood and can wipe them all down...cellular metabolism that is toxic to the cells), and need to be split very soon. There is power in numbers...or contaminated. This also ensures that anyone needing new cells in the lab can take a vial of the ‘mint...

...genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates and sequencing... such a scale, automation is crucial, both for speeding things up and for standardizing the process. When...mutates HDR donor templates to avoid re-cutting when needed. It gives you control over your data. CrispyCrunch...

...assembly levels were indeed possible, with correct, in-frame assembly proceeding in 99% of 12-fragment...assembly that would result in a blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test ...

...binding domains forming a building block sufficient to seed polymer formation as fusion protein concentration...designated mEGFP, mVenus, etc. A word of caution is needed because FPs not derived from GFP, i.e. the red ... FPs Find the Best Fluorescent Protein for Your Needs Get Tips for Using FRET in Your Experiments Additional...

... Cloning easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements...s really a matter of choosing the parts that you need from the kit and getting started making your own...They just start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working...

...terminals, which are jam packed with everything needed for synaptic transmission, are right around 250...Microscopy (EM) is one option, but the equipment needed for EM is not something you find in the average... don’t have access to the specialized equipment needed for EM or SRM? Enter Expansion Microscopy. What...

...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their...genuine interest to other researchers in their field. Indeed, sharing results with the broader scientific community...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...

...of a donor DNA molecule. Your donor molecule will need to have homology to the targeted locus on either...your donor molecule is used as the template, you need to incorporate homologous sequence to the right ...larger knock-in (200 bp – 2 kb), a dsDNA donor will need to be used due to synthesis limits of oligonucleotides...

...are planning, cost, and the amount of protein you need to produce.  The most common yeast strain used for...processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction coefficient to calculate ...

...have the annoying tendency to runout right when you need them and to accumulate a bunch of unused buffers... can still reduce the number of spin columns you need to complete your research. Do you know of other ...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

...does not drop significantly until protein levels exceed 70% of the sample content (Loughrey and Matlock...2019). To confirm RNA in your DNA sample, you will need to use a complementary assay, such as a fluorescence...readings are inherently more variable. If you do need to work with dilute samples:  Take multiple readings...

...race, it highlights the myriad issues researchers need to reflect upon before they attempt to make essentialist...of a true correlation.   It turned out there was indeed a bias, and it was coming through an unexpected...screens, they had also found the information they needed to begin correcting the issue. Working with James...