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We narrowed to 546 results for: EPO

Showing: 301 - 320 of 546 results
  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...
  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...sensors are better classified by their fluorescent reporter and the mechanism of their voltage sensing component...genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging viruses...
  3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....
  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...
  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...
  6. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods for various...or cell lines, please consider sharing them by depositing with Addgene! Figures were adapted from: Hall...
  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create...Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...
  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...experimental settings. It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER Center...Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity ...
  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining...
  10. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...(Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation of...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types, we hope...
  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...
  12. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...
  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    .... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...
  14. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...
  15. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Today, it'...or inaccessible. The Ting lab recently made two deposits that increase the utility of this technique.  ...
  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...
  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ... plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  Here's ...antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies...
  18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...
  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL...
Showing: 301 - 320 of 546 results