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We narrowed to 924 results for: Gene

Showing: 301 - 320 of 924 results

  1. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... researchers with many different ways to control gene expression. Get the full scoop from Addgenie A. ...how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6. CRISPR 101: Non-homologous...Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have limited packaging capacity making...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...them a read if you’ve missed them! Breakdown of Addgene's top blog posts from 2015 Our most popular posts...your own guest posts by e-mailing us at blog@addgene.org. Thank you again for a fantastic year and happy...

  2. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers, so Outreach Scientist...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As ...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...

  3. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation should...and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The...considerations you should think about when developing optogenetics behavioral experiments. There are far too many...many behaviors that have utilized optogenetics to be fully summarized in a short blog post, but some examples...ill-defined region of the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type...the literature to find all the applications of optogenetics relevant to your field. Ultimately, your question...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... development of optogenetics, has genetically manipulated a number of the opsin genes to alter the light...have also been engineered to express various opsin genes [7-9]. The advantage of using these lines is, if...Regardless of the method of expression of your opsin gene, it is essential to test the expression of your ...PMID: 22470147.  6. Gore BB, et al. Manipulating gene expression in projection-specific neuronal populations...sections of optogenetics papers. Optogenetics step one: Deciding how you want to use optogenetics The most...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and, ...electrophysiology to prove that your optogenetic manipulations are generating action potentials (or lack there...

  5. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...Hey guys, Blugene here! As Addgene’s mascot, I’ve watched Addgene grow into the successful, internationally...to the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the... the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and...changes at Addgene over the last 10 years as well as the many people that make up the Addgene family. It...Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination...make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

  6. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    .... Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an...pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating ...performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current...adult rat brain depending on the transduced area. Gene Ther. 2003 Feb;10(4):337-47. PubMed PMID: 12595892...Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...accelerate their work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses...please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools,...

  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering ...reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition of standards...antibiotic resistance markers, promoters, and inducible gene expression systems in bee gut microbiome species...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...address these questions, biologists often use next-generation sequencing. Sequencing the 16S rRNA reveals the... also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take a look ...microbiome with sequencing is vast, the ability to genetically alter microbiome members is limited and many ...

  8. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ... PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl;...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...

  9. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... your antibody is validated! Genetic Strategies This approach uses a gene knock-down or knock-out approach...all sample types are amenable to genetic manipulation, nor can all genes be easily knocked down or out. ...bioinformatician) to look at RNA expression of your gene of interest across a set of human tissues.  From...Conveniently, you found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9...plasmid encoding your protein of interest, known as Gene of Interest (GOI), and transfect it into your favorite...this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run a Western... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...

  10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning Sytem to Clone Your Gene of Interest...making it difficult to quickly identify possible gene function. In this post, I will describe a useful...done, this technique can also help pinpoint the genes within a sequenced genome that encode for functional...digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these fragments can be ...results in optimal likelihood of capturing a whole RBP gene without causing issues associated with cloning large...back to the original plate to identify which phage gene encodes that protein! First, block your membranes...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...

  11. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    .... Current table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated...reviewed articles. If you’re targeting your favorite gene with CRISPR, using one of these validated gRNAs ...is worth trying alternate names, particularly for gene names. If you find a sequence that will work for... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop... is welcomed for validated gRNAs that are not generated from a plasmid. If you, for example, prefer to...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...

  12. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    .... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...or to answer any questions you might have about Addgene. The page is divided into three main sections: ... real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their career paths, future...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...comments section below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom...

  13. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...their mission. Addgene is fortunate in that we are able to fulfill our mission by generating revenue through... is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution)... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  14. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...

  15. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...website Watch Addgene's video collection ...

  16. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

  17. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...travel award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

  18. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...
Showing: 301 - 320 of 924 results