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Showing: 301 - 320 of 878 results

  1. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...Welch says, “[It] seems counter-intuitive, but I find office work more mentally challenging than lab work... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...your time. As my colleague Mike Lemieux told me, “I find that at a desk, it is much easier to stick to timelines...who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly basis. ...amazing thing to be a part of and really helps me get behind a project or initiative.” If you think the meetings...science in the first place. Finally, if you still find the idea of not holding a pipette again upsetting...the community (and sometimes conduct their own independent research!). There are also organizations like...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Resources on Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make ...2017 RSpace was developed with these principles in mind, and in response to further input provided by the...repositories. Below I describe how each of these kinds of connectivity facilitates reproducibility. Connectivity...Slack conversations and import them into RSpace. The kind of informal conversations about research that can...enables users to conveniently select and deposit individual documents or more complex datasets into the desired... edit to particular groups and/or particular individuals, the issue of third-party access to the ELN arises...rory_macneil.     Additional resources on the Addgene blog Find tips on choosing an electronic lab notebook Watch...

  3. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...PUF Assembly Kit makes it possible to devise RNA binding proteins to hit any target of interest. The new... to human proteins known as Pumilio/fem-3 mRNA binding factors (PUF). In a single step, researchers can...domains for RNA specificity engineering. “The RNA binding domain is interesting because by changing certain...proteins include eight modular repeats, each of which binds a consecutive base in its RNA target. By changing... acids, it’s possible to produce a protein that binds an alternate sequence. By comparison, Abil explained...explained, the specificity of other RNA binding domains depends on the secondary structure of RNA. As a result... are endless.” Abil says the new tool might even find therapeutic application in addition to their use...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...Expression Kit FREQ Seq PUF Assembly Kit Below you’ll find information about what these kits contain, how they...Biological Chemistry 290.8 (2015): 4728-4740. You can find more info on pCri in Mary Gearing’s blog post on...translation initiation sequence known as the Species-Independent Translational Sequence (SITS). This sequence ...composed of repeat segments that can be engineered to bind specific RNA bases. By swapping out these different...repeat segments, you can create a PUF protein that binds your RNA sequence of interest. The Zhao lab has ...contains all the variant PUF segments required to bind any 8 base RNA sequence. The segments are provided.... Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil et al., 2014...

  5. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... are equal. Created with BioRender.com. This kind of normalization is a nearly ubiquitous tool, but... assumptions that their loading controls are independent of these other sources of error. It’s important...housekeeping protein in your context. If you don’t find anything, you’ll need to conduct a control experiment...strongly influenced by unexpected variations in any individual protein. This ensures you are truly comparing...with β-actin (1:2000). Representative of three independent blots. B–C) Graphs showing the relative intensity...increasing concentration of β-actin in panel A. Indeed, the quantification in panel B is not especially...total protein are compared. While a higher slope indicates higher sensitivity, linearity is the most important...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is...leading to counter-selection escape). The rhamnose induction system is well-characterized and quite tight (...placed the toxin genes under control of a rhamnose-inducible promoter, though Khetrapal et al. also demonstrated...that the arabinose promoter worked too. Rhamnose induction relies on the transcriptional activator rhaS. ... copy of rhaS with a strong synthetic ribosome binding site (using the online calculator from the Salis...are real! Planning an allelic exchange experiment? Find the pTOX plasmids here! Continued development of...the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...researchers are on the line. With these priorities in mind, many researchers go to great lengths to keep accurate...efficiently accomplish our daily goals. These tools remind us of our daily tasks, make our days more effective...effective, in both academic circles as well as in industry. The news is good. In their ideal form, ELNs provide...budgets, “a large percentage of survey respondents indicated that cost was a significant barrier to ELN adoption...to digital for example): “… 99% of respondents indicated that ease of use would influence their ELN choice... of it all in paper notebooks. Following these findings, we defined the priorities for future ELN development...schedule online meetings with the support teams behind them.  Conclusion Researchers spend 40% of their...

  8. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...feedback when it is merited. Surprisingly, some people find this hard so get used to it by making it a concrete...However, I have also found that scientists seem to find it easier to give feedback in formal sessions using... Here are the 3 things that worked least well. Find out the Effect of the Feedback: How does this feedback...over, what would you do differently? Beware of indirect language You must be honest about performance ... mistakes I see is managers who hint at or use indirect language to talk about real performance issues...issues. Watch this little video to learn more about indirect language from Steven Pinker. As a manager you ...and clear. There is place in human discourse for indirect language, but it is not the way to go when giving...

  9. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...-4 colonies to ensure that you find the correct product. Once you find a colony with the appropriately...Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional Protocols Browse Our... Some of these include, TA cloning, ligation independent cloning, TOPO cloning, one step cloning, and ...Li, et al., in 2011. This method is ligation independent, it does not require purification of insert or...simplified, purification-free, sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (...

  10. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple...mental freedom. Practical approaches Engaging in mindful meditation, taking field trips, and spending time...gain fresh perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration...Serendipity is not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative...successful research career, whether within academia or industry. Albert Einstein once said: “There are only two...serendipity into your research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking...

  11. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...s-level job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is ...work something you can see yourself enjoying?  To find master’s-level postings, try adding “master’s” to...filters to limit results to people that work in industries you’re interested in (i.e. biotechnology, pharmaceuticals...switch your field of work, both in academia and in industry. Advancing along a career path in these two fields...fields looks different however. In industry the career ladder is larger and there are more opportunities...Career advancement for PhDs, both in academia and industry, often means moving from the lab to a desk, although...on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the Science Career...

  12. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...to be binding at particular genomic sites when in fact they are just non-specific or indirectly interacting...is the go-to method for mapping where a protein binds genome-wide, and has been widely applied in many..., an enzyme-based protocol for identifying DNA binding patterns that overcomes some of the limitations...Protein A fused MNase is then added and Protein A binds Immunoglobulin G (IgG) thus targeting MNase to antibody...provides an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data...with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for ...

  13. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...emits red and green fluorescence. Following the induction of bulk autophagy, Rosella is targeted to the ...interest to track the autophagy of that protein. Find the cytosol-targeted and mitochondria-targeted Rosella...nm to 440 nm serves as a measure of autophagy induction: when Keima is in the cytosol, this ratio is low... this biosensor can’t be used with fixed cells. Find the mitochondria-targeted Keima variant at Addgene... in yellow puncta but a decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3...fluorescence suggests a decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References...

  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...still invested in seeing how they do it.    Finding Conflict in Science  In all science communication...conflict into your work, but one of my favorite is to find it within the science itself - within that process...trying to understand your completely unexpected findings, or understanding why your experiment has not ...not worked…again…for the seventh time….   I find that once I sit down and talk to a scientist about their...paper was actually from a completely unexpected finding in a different experiment, this tool was developed...fodder for science communication. The trick is to find one or two roadblocks that sparked creativity, changed... a story around them. A good rule of thumb is to find the pieces that make you think, “Oh, that’s interesting...

  15. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...for scientists working at home, and you will still find this useful. Now that we’ve been doing this for ...safe space. No matter why, now is a great time to find a few colleagues to work together on career and ... “the ability to assess and initiate things independently” and “the power or opportunity to act or take...format is good for everyone including those that find events stressful. Video chatting makes it pretty...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you are in ..., your PI is likely working on a number of them. Find one to apply to or offer to help with lab grant ...Opportunities Open Education Database: 100 Places to Find Funding For Your Research University of Florida:...

  16. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...web-based, career-planning platform called the Individual Development Plan (IDP) and uses it to match qualified... qualified scientists to jobs in industry, academia, and government. This great resource allows the undecided...and other professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an...interests: Use career planning tools such as the Individual Development Plan to generate a list of potential... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...potential job Join LinkedIn and other job sites to find open positions and view their required qualifications...

  17. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...single peak, indicating one amplicon and no primer dimers. (b) A shoulder peak indicating primer dimers...the right conditions and parameters, some people find it can detect even smaller differences, and it can...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...different types of pipettes and pipetting methods to find what’s most consistent in your hands.  When using...target in the reaction should be compared to an individually run sample when developing the protocol and ...and optimized until the multiplexed and individual values match across the board. If the samples or sample... e-book on applying MIQE guidelines! With these kinds of resources, you will soon become a qPCR master...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...targeting and sequencing millions of cells. For every individual sgRNA, you recover a minimum of 1,000 targeted... 5% off-target frequency is something to keep in mind, but likely will not have a significant impact on... many predictive online tools which can not only find gRNAs for you, but also score their probability ...probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such software typically only...at mismatched guide sites, but not necessarily binding to these sites. Thus, these enzyme swaps will only...need to do your due diligence to ensure they are indeed a non-issue. Above we mentioned how to predict ...tested with some success. The major con is that binding by Cas does not always correlate with catalytic...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...expected fragments for that plasmid. This phenomenon indicates the presence of a multimer.   Fig. 1:...multimerization has been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins... al., 2010; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide...multimerization. One study by Bacolla et al. (2011) indicated that strain HB101 produced the highest yield of... at reducing multimerization and define recA-independent mechanisms. Avoid overgrowing the bacterial culture...-B DNA-forming sequences and WRN deficiency independently increase the frequency of base substitution ...26104344. Fishel RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...seamless manner, leaving no sequences or mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying...cells lines, including stem cells, without leaving behind any accessory DNA sequences [2], [3]. Simply put...integration [4], the donor cassette is removed leaving behind no accessory transposon sequences.  The use of ... plasmid after excision by PBx, thereby leaving behind only those cells with a Footprint-Free™ genome ...this method can be effective, a loxP site is left behind in the genome following donor removal, which alters...somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. With the ...Cathomen, and K. Schwarz, “Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: wanted ...
Showing: 301 - 320 of 878 results