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We narrowed to 889 results for: IND

Showing: 301 - 320 of 889 results

  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...experiments in labs around the world were put on hold indefinitely due to COVID-19. In our lab, we were interested...placing orders for paper x on date t. We cannot use individual plasmid orders, because when a PI or lab places...several plasmids at once, breaking the statistical independence assumption of the Poisson process. This modification... by COVID-19, so we defined the COVID-19 event windows as 01/15/20 -- 03/15/20 for China and 03/11/20 ... The inferred parameters suggest that COVID-19 induced a 2x–5x reduction in the rate of scientists placing...more dramatic than the COVID-19 effect, though we remind the reader that this is not a causal interpretation... In conclusion, our model infers that COVID-19 induced a 2x–5x reduction in the rate of scientists placing...

  2. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...you’ve thought about starting a journal club is to find a topic and seeing if others are interested. You...run the journal club. Some are led by a single individual who walks through the paper figure by figure....single presenter summarizing the paper’s major findings and then focusing more on broader implications...implications rather than diving deep into the individual data points. All formats should be an open forum where ... attend for scheduling conflicts. This helped us find a time that accommodates more people. The feedback...very point is discussion. Perhaps the project is winding down. Perhaps other projects have taken on a larger... often driven by one or two participants. If you find yourself in this situation, considering stopping...

  3. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...it lacks Cas9’s other HNH endonuclease domain, indicating that Cpf1 functions differently. Since Cpf1 loci...red) or LbCpf1 (blue). Mismatch positions are indicated below each graph. Figure from Kleinstiver et al... and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing in a cell culture model. The...CRISPR-Cas system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche...Biotechnol. 35.1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim.... Nat Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver... Nat Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova...

  4. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...Erhardt S, Lindskog M, Ruas JL. doi: 10.1016/j.cell.2014.07.051. PMID: 25259918 Muscle over mind. Moon HY...transgenic mice that helps to explain how it works. The findings in a recent issue of Cell demonstrate how PGC-...PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance, protects the...in the body, the brain is protected from stress-induced biochemical changes that could otherwise lead to...of the kidney or the liver.” The discovery is a window onto communication between the muscle and brain...kynurenine metabolism and mediates resilience to stress-induced depression. Agudelo LZ, Femenía T, Orhan F, Porsmyr-Palmertz...

  5. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...Welch says, “[It] seems counter-intuitive, but I find office work more mentally challenging than lab work... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...your time. As my colleague Mike Lemieux told me, “I find that at a desk, it is much easier to stick to timelines...who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly basis. ...amazing thing to be a part of and really helps me get behind a project or initiative.” If you think the meetings...science in the first place. Finally, if you still find the idea of not holding a pipette again upsetting...the community (and sometimes conduct their own independent research!). There are also organizations like...

  6. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Resources on Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make ...2017 RSpace was developed with these principles in mind, and in response to further input provided by the...repositories. Below I describe how each of these kinds of connectivity facilitates reproducibility. Connectivity...Slack conversations and import them into RSpace. The kind of informal conversations about research that can...enables users to conveniently select and deposit individual documents or more complex datasets into the desired... edit to particular groups and/or particular individuals, the issue of third-party access to the ELN arises...rory_macneil.     Additional resources on the Addgene blog Find tips on choosing an electronic lab notebook Watch...

  7. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...cancer. These later stages of a cancer diagnosis indicate, for Stage 3, that the cancer is large in size...size and may have spread while Stage 4 indicates that the cancer has spread from where it originated to ...if not all) of the cancer cells that may be left behind after surgery, while being less traumatic to the...into the cytoplasm, migrates out of the cell, and binds cells that can begin an immune response. This important...and even toys! Contrary to the belief of many individuals, asbestos has not been banned in the United States...such a ban, and the president of the organization, Linda Reinstein – whose husband passed away from mesothelioma...Alliance. Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection...

  8. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...PUF Assembly Kit makes it possible to devise RNA binding proteins to hit any target of interest. The new... to human proteins known as Pumilio/fem-3 mRNA binding factors (PUF). In a single step, researchers can...domains for RNA specificity engineering. “The RNA binding domain is interesting because by changing certain...proteins include eight modular repeats, each of which binds a consecutive base in its RNA target. By changing... acids, it’s possible to produce a protein that binds an alternate sequence. By comparison, Abil explained...explained, the specificity of other RNA binding domains depends on the secondary structure of RNA. As a result... are endless.” Abil says the new tool might even find therapeutic application in addition to their use...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is...leading to counter-selection escape). The rhamnose induction system is well-characterized and quite tight (...placed the toxin genes under control of a rhamnose-inducible promoter, though Khetrapal et al. also demonstrated...that the arabinose promoter worked too. Rhamnose induction relies on the transcriptional activator rhaS. ... copy of rhaS with a strong synthetic ribosome binding site (using the online calculator from the Salis...are real! Planning an allelic exchange experiment? Find the pTOX plasmids here! Continued development of...the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red...

  10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...researchers are on the line. With these priorities in mind, many researchers go to great lengths to keep accurate...efficiently accomplish our daily goals. These tools remind us of our daily tasks, make our days more effective...effective, in both academic circles as well as in industry. The news is good. In their ideal form, ELNs provide...budgets, “a large percentage of survey respondents indicated that cost was a significant barrier to ELN adoption...to digital for example): “… 99% of respondents indicated that ease of use would influence their ELN choice... of it all in paper notebooks. Following these findings, we defined the priorities for future ELN development...schedule online meetings with the support teams behind them.  Conclusion Researchers spend 40% of their...

  11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... are equal. Created with BioRender.com. This kind of normalization is a nearly ubiquitous tool, but... assumptions that their loading controls are independent of these other sources of error. It’s important...housekeeping protein in your context. If you don’t find anything, you’ll need to conduct a control experiment...strongly influenced by unexpected variations in any individual protein. This ensures you are truly comparing...with β-actin (1:2000). Representative of three independent blots. B–C) Graphs showing the relative intensity...increasing concentration of β-actin in panel A. Indeed, the quantification in panel B is not especially...total protein are compared. While a higher slope indicates higher sensitivity, linearity is the most important...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...Expression Kit FREQ Seq PUF Assembly Kit Below you’ll find information about what these kits contain, how they...Biological Chemistry 290.8 (2015): 4728-4740. You can find more info on pCri in Mary Gearing’s blog post on...translation initiation sequence known as the Species-Independent Translational Sequence (SITS). This sequence ...composed of repeat segments that can be engineered to bind specific RNA bases. By swapping out these different...repeat segments, you can create a PUF protein that binds your RNA sequence of interest. The Zhao lab has ...contains all the variant PUF segments required to bind any 8 base RNA sequence. The segments are provided.... Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil et al., 2014...

  13. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...s-level job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is ...work something you can see yourself enjoying?  To find master’s-level postings, try adding “master’s” to...filters to limit results to people that work in industries you’re interested in (i.e. biotechnology, pharmaceuticals...switch your field of work, both in academia and in industry. Advancing along a career path in these two fields...fields looks different however. In industry the career ladder is larger and there are more opportunities...Career advancement for PhDs, both in academia and industry, often means moving from the lab to a desk, although...on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the Science Career...

  14. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple...mental freedom. Practical approaches Engaging in mindful meditation, taking field trips, and spending time...gain fresh perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration...Serendipity is not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative...successful research career, whether within academia or industry. Albert Einstein once said: “There are only two...serendipity into your research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking...

  15. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...to be binding at particular genomic sites when in fact they are just non-specific or indirectly interacting...is the go-to method for mapping where a protein binds genome-wide, and has been widely applied in many..., an enzyme-based protocol for identifying DNA binding patterns that overcomes some of the limitations...Protein A fused MNase is then added and Protein A binds Immunoglobulin G (IgG) thus targeting MNase to antibody...provides an excellent additional measure of DNA binding sites in combination with existing ChIP-seq data...with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for ...

  16. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...-4 colonies to ensure that you find the correct product. Once you find a colony with the appropriately...Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional Protocols Browse Our... Some of these include, TA cloning, ligation independent cloning, TOPO cloning, one step cloning, and ...Li, et al., in 2011. This method is ligation independent, it does not require purification of insert or...simplified, purification-free, sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (...

  17. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...feedback when it is merited. Surprisingly, some people find this hard so get used to it by making it a concrete...However, I have also found that scientists seem to find it easier to give feedback in formal sessions using... Here are the 3 things that worked least well. Find out the Effect of the Feedback: How does this feedback...over, what would you do differently? Beware of indirect language You must be honest about performance ... mistakes I see is managers who hint at or use indirect language to talk about real performance issues...issues. Watch this little video to learn more about indirect language from Steven Pinker. As a manager you ...and clear. There is place in human discourse for indirect language, but it is not the way to go when giving...

  18. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...emits red and green fluorescence. Following the induction of bulk autophagy, Rosella is targeted to the ...interest to track the autophagy of that protein. Find the cytosol-targeted and mitochondria-targeted Rosella...nm to 440 nm serves as a measure of autophagy induction: when Keima is in the cytosol, this ratio is low... this biosensor can’t be used with fixed cells. Find the mitochondria-targeted Keima variant at Addgene... in yellow puncta but a decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3...fluorescence suggests a decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References...

  19. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...for scientists working at home, and you will still find this useful. Now that we’ve been doing this for ...safe space. No matter why, now is a great time to find a few colleagues to work together on career and ... “the ability to assess and initiate things independently” and “the power or opportunity to act or take...format is good for everyone including those that find events stressful. Video chatting makes it pretty...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you are in ..., your PI is likely working on a number of them. Find one to apply to or offer to help with lab grant ...Opportunities Open Education Database: 100 Places to Find Funding For Your Research University of Florida:...

  20. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...still invested in seeing how they do it.    Finding Conflict in Science  In all science communication...conflict into your work, but one of my favorite is to find it within the science itself - within that process...trying to understand your completely unexpected findings, or understanding why your experiment has not ...not worked…again…for the seventh time….   I find that once I sit down and talk to a scientist about their...paper was actually from a completely unexpected finding in a different experiment, this tool was developed...fodder for science communication. The trick is to find one or two roadblocks that sparked creativity, changed... a story around them. A good rule of thumb is to find the pieces that make you think, “Oh, that’s interesting...
Showing: 301 - 320 of 889 results