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We narrowed to 791 results for: Kin

Showing: 301 - 320 of 791 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that ...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s...initiated at a Hawaiian deli in 1972. Using a deli napkin for paper, a small group of scientists including... curate, and distribute them all in the name of making it a little bit easier for scientists to conduct...

  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond...players in the game (even beyond K. phaffii ) worth looking into whether you’re just dipping your toes into...

  3. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped... the key points you’ll need to remember when undertaking library amplification and sequencing. Maintain...

  4. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Will the job require public speaking? Will you be leading a group or working as part of team? Will you .... It is all too easy to get tunnel vision when working towards a specific goal, and when you realise that...often be misleading. Key aspects to determine are working hours (9am-5pm Mon-Fri or overtime and weekends...

  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...will define positive selection as the means of picking up positive traits (i.e. the plasmid contains a...toxin), whereas negative selection would be the picking up of a negative trait (i.e. the plasmid contains...Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3...transfection when creating stable cell lines. Looking for Mammalian Expression Plasmids? Check Out Addgene's...

  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify...components: the error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error...error-prone polymerase (PolI) that is fused to a nicking Cas9. A guide directs Cas9 to a site of interest...changes promote nCas9’s dissociation from DNA after nicking and yielded an enhance nCas9 (enCas9) EvolvR with...

  7. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... with translatable skills: working with data, working with people, working with complex processes Arm ...Ginkgo Bioworks, our software development team was working on lab operations software for next-gen sequencing...

  8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...spread of COVID-19. That’s why we’re proud to be working with Ginkgo Bioworks to synthesize and share over...analyzed first thing the following day. Nothing like waking up to fresh data! These same plasmids are then ...ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared to our standard...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

  9. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...gate which selects anything to the right of it, marking it as GFP+. Quantification for such graphs could...plot! The dreaded smear - fear not! When you are looking to study the level of protein expression, instead...Figure 2). These smears tend to occur when you are looking at proteins that are expressed regularly in cells...percentage positive or negative in your dot plot, looking at a histogram vs antibody plot would be better...

  10. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... the experiment in my head, double-checking details, or just taking a moment to relax. During really stressful...worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly. Have ...however, many ways for you to reduce the chances of making an error. Try a variety of things, including (and...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...question more and more students are asking. Teachers are also asking, “How do I engage my students in real-world...outcomes by emphasizing the importance of critical thinking, persistence, teamwork, and communication. And... the only informal synthetic biology club of its kind, running from start to finish during the academic...

  12. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...work best for your scientific question? If you’re thinking, ‘wait, those terms mean different things?!’, ... to clearly define what type of factor you are looking for and what process or outcome that factor functions... advantage – that is, you are not specifically looking at cells that can survive. Instead, all manipulations... screens are similar to CRISPR, but instead of knocking out the genes of interest (or the whole genome...

  13. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, ...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing, and an...include in my paper? More and more journals are asking for detailed information on lab resources, such...enough information to replicate your experiments, making research more reproducible and your results more...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming... pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and... injecting iPSCs into immunodeficient mice and looking for tumors that contains cells from all three germ...of iPSCs is the number one priority, with safety taking lower priority. Genomic integration is less of ... and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent stem cells: reprogramming à...

  16. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...factor, vascular endothelial growth factor, and interleukin-6 (Witkiewicz et al., 2009) which contribute ...metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy cell type in the...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

  17. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...like cross-linking immunoprecipitation (CLIP) identify RNA-protein interactions by cross-linking RNAs and...enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used...RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help answer? Ramanathan et al...

  18. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids...of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were...CRISPR editing. Conclusions & implications One striking feature of this paper is the failure of the embryos...comprised of individuals with varied backgrounds. Talking about this study with non-biologist friends Like...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation of pre-autophagosomal...an autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about...easier to study the mechanisms behind autophagy by tracking what’s being transported to the yeast vacuole ...vacuole (i.e. cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful...

  20. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...the design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences...their recognition sequence, creating four base flanking overhangs. Since these overhangs are not part ...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...
Showing: 301 - 320 of 791 results