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Showing: 301 - 320 of 676 results
  1. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...tribulations of the modern PhD program. Don’t be fooled! Most senior graduate students are friendly and full of...University of California, Los Angeles. She enjoys chromosome biology and reading Ben Vincent's tweets.    ...
  2. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A [email protected] if you are interested. Addgene's Most Popular Posts of 2014 How to Design Your gRNA...
  3. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...CRISPR plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably...from 231 countries. Guides and protocols were our most popular content, while our beloved blog received...
  4. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the...year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...
  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...of nonacademic science careers is that a team is almost always involved. Demonstrating your ability to ...
  6. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ... the kit in December, GreenGate includes the six most frequently used elements in plant expression cassettes...generate multi-construct transgenic plants. In the most practical terms, what GreenGate can do is “save ...
  7. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...feedback from the team. Successful management can almost be boiled down to one, key concept: Creating a ... style.   Seeking feedback frequently One of the most important paths of information flow is the one that...
  8. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone. They...requires an awful amount of skill,  time for which most researchers wouldn't have. They are motivated to...
  9. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells using zinc finger nucleases...
  10. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...communicate exactly what a site offers to their users, and most importantly, allow those users the ability to quickly...upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile, but occasionally you might...
  11. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... of information to fit into a single plasmid, so most first-generation binary vectors are quite large ...promoter. Originally isolated from the Mirabilis mosaic virus, the constitutively active M24 promoter has...
  12. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...they are peptides which do not naturally exist in most expression systems that can be added to the N or...downstream applications Below, we describe a few of the most common tags available. Maltose binding protein Maltose...
  13. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics... be very informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to ...
  14. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...display protein aggregation, it’s recommended for most imaging applications. What can you do with SunTag... experiments in their paper, and so can you! In almost any case you would use a traditional FP fusion,...
  15. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...advancing the field.   What is a plasmid? At their most basic level, plasmids are small circular pieces ...that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria, but also...
  16. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...Montgomery, or Louis Miller led these sessions. The most beneficial aspect of this course was breaking up...works.  4. Practice writing everyday Overall, the most important instruction the classes taught me was ...
  17. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...levels of an organization and is also one of the most common criteria required for a promotion. Yet little...fulfilling the requirements of the current task. Most of us start as doers, some become managers and some...
Showing: 301 - 320 of 676 results