We narrowed to 329 results for: NUC

1. Viral Production at Addgene

   Type

   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

2. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...DnaA-ATP in initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi....

3. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

4. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively ...

5. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ... "Excitatory transmission from the amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356...

6. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...small RNA expression shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, ...

7. Five Popular Model Organisms

   Type

   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ...like those that overexpress the toxic protein α-synuclein (the most common protein found in lewy bodies)...

8. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the genome that...

9. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ...by Rho family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner...

10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...to specific subcellular locations, such as the nuclear envelope, allowing scientists to probe interactions...

11. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature...

12. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...PhosphoSitePlus, 2014: mutations, PTMs and recalibrations." Nucleic acids research 43.D1 (2015): D512-D520. PubMed ...

13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...this approach doesn’t require a functional Cas9 nuclease, so there’s no potential for aberrant DNA cleavage...

14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...inversion of the construct. Sanes and Lichtman used pronuclear injection to insert these Brainbow constructs...

15. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...2025). In retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking...

16. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...compartments such as the mitochondria, cytoplasm and nucleus.  We hope that this blog has provided you with ...

17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ... in order to protect their DNA from host cell nucleases (4). To further complicate matters, even when ...

18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...result interpretation. Also, the signals from the nuclear area make the detection of mycoplasmas difficult...

19. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...LTRs) has been removed, Rev (which facilitates nuclear export) is expressed from a separate plasmid, and...

20. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ... Nicholas Thompson is a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic...