We narrowed to 347 results for: SAM-2

...) 12 (>100) xCas9(3.7)-ABE 69 (1.4) 43 (2) 21 (3) Not examined 16 (>100) Average percent...mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When they ...Adenine base editor xCas9 3.7-ABE also follows the same pattern, with improved editing at an NGG PAM (69...

...PMID: 25538079. PubMed Central PMCID: PMC4353587. 2. Stephen, Andrew G., et al. "Dragging ras back in ...brake pedal that does not work. Both cases yield the same result – uncontrolled movement of the vehicle or...transcript most commonly found across all TCGA tumor samples is represented, making the results of any such ...

... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange... on these and any other CRISPR screens using the same reference genomes and libraries. Further complicating...

... replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through insertional...often: Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral ...both lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), ...

...this year. Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains... For an N-terminally tagged protein, we used the same strategy, inserting the tag and the linker preceding...

...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which...repair is a random process, zygotes edited using the same nuclease may not result in genetically identical...

...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...amino acids and three stop codons, meaning that the same amino acid can be encoded by more than one codon...organisms. There are several codons that encode the same amino acid. Each organism, however, may preferentially...

...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...plasmids, all of which need to be represented in the same proportion after amplification as they were prior...visualization) that confirm diversity of the library sample, and any other information or scripts provided ...

...Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells." State University...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...

...run as long as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments... replicates are repeated measurements of the same sample and are included to assess variability in the... the tau western blot several times with the same samples and compare the results across runs. If the ... wild-type and knockout samples. In this test, wild-type and knockout samples are treated with the identical...present in the wild-type samples and absent in the knockout.  If knockout samples are unavailable, try substituting...interactions between the sample and the secondary antibody. C, a label control treats the sample to the full protocol...interpretation of results. A positive control is generally a sample or group that will have a desired response. For...

...C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to ...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate...

...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...requires lots of data. Get your community involved in sampling or bioinformatics analysis or upload your data...

...systems to defend against both DNA and RNA viruses - 2 cas systems regulate at the DNA level and 1 at the...from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’s Ribobio...

...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...population within a given tissue rather than a mixed sample. TRIBE was developed in Drosophila, and further...

... Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed... with a knockout and expression construct on the same piece of DNA. We do a lot of protein-protein interaction...

...  Figure 2: Examples of simultaneous multiplex IF labeling of rat brain samples from the James ...presence of several biomarkers together in the same tissue sample predicts a certain cancer and want to look...you will need to start with a fixed sample. Depending on the sample type, this might be frozen or formalin-fixed...al, 2022 for a sample protocol.)  Simultaneous IF Depending on your needs, your sample, and targets, you...known to be abundant in your samples (with a primary antibody that has the same species and isotype as the...set of samples where you label only one target in each. Compare these individually-labeled samples with ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC)...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward.... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli, two more...PMC5679246. Strack, Rita L., Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the...

..., can protect cryo-EM samples from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue...protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged...and structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered and evolved...antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination By Mike ...enrichment streamlines the purification and minimizes sample loss while also supporting imaging, making it easy...cryo-EM grid.     Figure 1: MagIC-cryo-EM for sample enrichment and structure determination. A) Schematic...beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https://doi.org...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...function like your average AAVs — and have many of the same restrictions, like limited cargo space (~4.7 kb)...systems, which use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads...efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly requested systemic...off-target organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction...