We narrowed to 611 results for: STO
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TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been working on ...given promoter. To harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive...
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Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. For your...until you have good separation between your bands. Stopping the gel when the bromophenol blue dye line is ... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance...would like to thank all of our depositors and requestors for making their reagents available and for supporting... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m.... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions...resources that answer questions our depositors and customers are asking. So how can you get involved? Keep ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....the frozen cell line becomes non-recoverable, the stock of the mAb becomes limited, which can be a threat...able to isolate RNA from non-recoverable hybridoma stocks, without having to grow up the cells. This RNA ... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....Great! But don’t just stop using it cold turkey. Step back gradually; maybe you stop to check things off... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....space offers STEM programming, workshops, and customized programs to meet the needs of students and teachers...blogger, Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....calcium events), Greater brightness, Less signal distortion from photoswitching or photobleaching Improved...addgene.org Fluorescent Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request ... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m.... tasksDelegation can feel unnatural for those accustomed to hands-on work. The temptation to "do it yourself...Matteo Tardelli authored the book "Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below! Additional resources ... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....involved in central anthocyanin metabolism are well understood. Additional work has also identified and characterized...how you were able to overcome them. Share your stories in the comments below! And if you have developed... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending this...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that...the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...interactions in cells because the protein binders can be customized for protein visualization, relocation, degradation... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away - an antibody-based... super-resolution microscopy.” Endosomes are historically difficult to study in living cells using conventional... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....refers to the work put into creating a permanent store of knowledge (a schema).” In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...