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Showing: 301 - 320 of 512 results

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...independent variable. Biological replicates In any given set of experiments, the biological controls are more ...

  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP tags can be recognized by distinct...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  11. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...antigen or an antibody depending on how the assay is set up. Then they block the capture reagent to prevent...binding to a primary antibody.  While the precise setup of the method can vary, typically users first coat...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...facilitates the use of prime editing in pooled settings with hundreds to thousands of pegRNAs. Chromatin-based...primer binding site, and many more. Once we had our datasets with all the different features and editing efficiencies...PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in the training of PRIDICT2.0 make this model...

  13. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators, which...packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...

  15. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...have become a powerful tool to address a diverse set of biological inquiries. References Ogura, Teru...death. The team used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed...for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be ... selects for those cells that have removed the cassette through a second recombination event between the...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...that do not express the protein. With this control set, you should see your target protein IP’d in the positive...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...
Showing: 301 - 320 of 512 results