We narrowed to 527 results for: Set;

...chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way they...

...Pathway Clone Collection 2.0. The collection is a set of vectors for use with the Gateway Cloning Platform...

...there include: Google Groups - I've specifically set up a forum where NgAgo users can share protocols ...

...a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately raises a...

...containing serum in each well. A 200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box...

...list of posters of interest, then you’ve already set yourself up for success. But sometimes, attendees...

... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting...

...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

... brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657,...

...working on one process at a time, and testing a small set of data each time a new process was added, we progressively...easily shared with others without any complicated setup. We created, tested, and debugged each of our pipeline...

...that do not express the protein. With this control set, you should see your target protein IP’d in the positive...

...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

... for saving time and avoiding potentially costly set-ups. However, there are many cases where the specific...

...discovered in 1856 by William Perkin. His discovery set off a flurry of activity and the majority of the ...

.... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

...it’s simple. The RT protein is made by a separate set of instructions in the same producer cells as the...

...sales-y”. The work includes reviewing customer data sets, troubleshooting protocols, analyzing information...

... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...