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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 612 results for: Sis

Showing: 301 - 320 of 612 results

  2. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at...advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

  6. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...clarification, meat tendering, and oligosaccharide synthesis. A major incentive to use cold-active psychrophilic...infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team...

  9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...can be (part of) figure 1 of your next paper or thesis. This series of posts explains how to critically...Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  10. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...Less than 20 ng of total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  11. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...

  14. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  15. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...have become a gold standard in gene expression analysis to investigate the effect of regulatory elements...you choose. Many luciferase assays require cell lysis as the most efficient means to disrupt the cell ...

  17. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

  18. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

  19. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  20. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...
Showing: 301 - 320 of 612 results