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Showing: 301 - 320 of 603 results

  1. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...Less than 20 ng of total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...

  4. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

  6. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...clarification, meat tendering, and oligosaccharide synthesis. A major incentive to use cold-active psychrophilic...infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

  10. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...

  13. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...and make up their own minds on whether the data analysis has been of good quality and whether they agree...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  15. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

  18. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...

  20. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...
Showing: 301 - 320 of 603 results