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We narrowed to 458 results for: Spr

Showing: 301 - 320 of 458 results

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...our “google doc of gratitude.” We must keep spreading the word about materials sharing, but we don’t... who support us by depositing, requesting and spreading the word. Our blog and site resources are just...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with...Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

  8. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... position the tips indepently making it easy to spread out your samples. This can be useful if you’re ...plates onto agar q-trays where the samples must be spread much farther apart before plating. With this fine...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...cascades, quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity...

  10. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...

  11. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...the best promoter to use, or if and how much the spread of the virus to adjacent areas impacted the experiments...NST. (A-C) Low-magnification images showing viral spread. (D-F)  High-magnification images immuno-stained...

  15. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...history. Interestingly, he also adds to the disproportionately large number of honorees to have trained ...Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects MDH-focused CUREs at...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https://...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...trials for prevalent diseases - to name a few. CRISPR-based tools are also particularly well suited to.... Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina. Front Cell ...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...later. The PNS presents a different problem: it’s spread throughout the body and is often difficult to surgically...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

  19. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we ...Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library...

  20. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...
Showing: 301 - 320 of 458 results