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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 642 results for: Vars;

Showing: 301 - 320 of 642 results

  1. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...great overview of the development of CRISPR into a varied research toolbox, as well as additional resources...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...

  3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase...

  4. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...into a vector, scientists may utilize one of a variety of cloning methods (restriction enzyme, ligation...research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid use...

  5. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  6. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced... Various Addgenies...

  11. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...Every worker at every level does better with some variety in their job. Good relationships with clients and...mental space and dislike intrusions (this actually varies, a manager does best to identify the work style...

  12. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...

  15. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification...

  16. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...improving the gRNA design process and rules for various applications is necessary going forward. The standardization...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

  18. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver nucleic...number of ready-to-use viral preps available for a variety of broadly applicable and popular viral vectors...

  19. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...system could be used to deliver or measure new variables. The sky is really the limit for this emerging...
Showing: 301 - 320 of 642 results