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Showing: 301 - 320 of 633 results

  1. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

  2. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...patterns (tissue/timing) can be monitored under various conditions without necessarily modifying the gene... are useful Traditional genetic screens employ various on/off switches to measure the function of a gene...

  3. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...

  5. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...Every worker at every level does better with some variety in their job. Good relationships with clients and...mental space and dislike intrusions (this actually varies, a manager does best to identify the work style...

  6. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...

  7. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...improving the gRNA design process and rules for various applications is necessary going forward. The standardization...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

  10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...PMC1698604. Ai, Hui-wang, et al. "Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification...

  11. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...the community about how the antibodies work in various applications, enabling you to better plan your ...antibody, the target antigen, and performance in various applications are included in the full webpage. ...

  12. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...plant cell culture), which is great for a wide variety of applications. But some techniques require you... RUBY plant. (Bottom panels) RUBY expression can vary from different transformation events likely due ...

  14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

  15. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...system could be used to deliver or measure new variables. The sky is really the limit for this emerging...

  16. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a variety of antibodies...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver nucleic...number of ready-to-use viral preps available for a variety of broadly applicable and popular viral vectors...

  18. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...management team). Sreyan is a research associate at Harvard Medical School and runs everything science (CSO... get through to a number of experts, including Harvard/MIT faculty, venture capital and angel investors...

  19. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... start somewhere. I knew if I started with the Harvard Business Review or some Chartered Institute of ...further education may allow you to transition into various realms of business.  Thank you to our guest blogger...

  20. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...resource allows the undecided to learn about a variety of science professions based on their skills and...future regulations impact your work? What design variables need consideration? Enrich your knowledge and ...
Showing: 301 - 320 of 633 results