We narrowed to 348 results for: abo.4

...histories, accurate to within 40 minutes. Figure 4: Time-resolved protein ticker tape. A) Design of iPAK4...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

...transcriptional activation screening. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 ...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

...just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’...interesting content Talk about something you know a lot about and that you care about. Don’t try to present...engaging and memorable. If you read my story above about the presentation faux pas, you are more likely...the room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs ...dynamite presentation?  To that end, let’s chat about giving science talks and how to make them serve ...other people give great talks We are not talking about lab meeting here. Lifeless presentation of experimental...You want to give the audience something to think about, argue with, or learn from. Some of the best talks...

...general mission to share- as opposed to sell- science. 4. You can prepare answers beforehand. Joanne answered...not too worried about selling yourself and really do just want to get information about yourself and your...where users can participate in forum discussions about a wide variety of topics. Individual forums are ...where users can ask designated scientists anything about a particular topic. Given Joanne’s expertise in... is well moderated. One of our original worries about sharing science advice on Reddit was that we’d be...answering questions. r/Science has pretty strict rules about not being too sales-y in posts which falls in line...any scientists to refer to if they have questions about science careers, women in STEM, work-life negotiation...

...Science Talk." 4. The chalk talk, simple in principle The first question I usually get about chalk talks ... potential colleagues study. Think about how you might collaborate or, at the absolute minimum, can appreciate...the first sentence or two what your research is about and what you think you can accomplish. No matter...your seminar accessible. Let the experts ask you about the details and then you can display your deep knowledge...expert's question than to leave everyone confused about your research because you waded too deep into the...obstacles, alternative approaches, potential collaborations, critiques of your postdoctoral research, etc...effectively convey your message. Key Things to Think about When Developing your Chalk Talk These items should...

...Plasmid incompatibility. Microbiology Reviews. 1987;51(4):381–395. Pubmed PMID: 3325793 Schumacher MA (2012...101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning Resources... plasmids within the same bacterial cell, think about whether plasmid incompatibility will affect your...this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now, bioinformatics...of the microbiological world. (Want to know more about these plasmid types? Read our Plasmids 101: Environmental...

...extremes lies the linear detection range. Figure 4: The linear detection range of the assay is between... this chain (blue) to infer or calculate things about earlier ones. In practice, multiple sources of error...condition 2.” By eye, the loading control appears about the same between the two conditions. But it isn’.... However, it throws both assumptions discussed above into question. Assumption #1: The loading control...searching the literature to see what is already known about your housekeeping protein in your context. If you...step from the POI blot, you don’t need to worry about the linear detection ranges of multiple targets....

...null clone, you should screen, at the very least, 4 clones. Did you use a dual guide system to generate... your edit, it’s important to know a few things about your target cells and the desired edits. First, ...et al., NAR). It works just like TIDE (outlined above), except that it uses a 3rd sequencing trace file...great option, especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing...higher probability locations. If you’re worried about off-target effects, you can consider using a version...SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with...

... 533.7601 (2016): 125-125. PubMed PMID: 27120160. 4. Kwart, Dylan, et al. "Precise and efficient scarless...mutations When thinking further about the distance relationship described above, we realized that, in addition...efficiency already drops by half and after only about 30 bp, it is no longer feasible to incorporate mutations...Additional Resources on the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your gRNA...

...PMID: 24463574. Pubmed Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic...following unanswered questions about CRISPR screen design. Unanswered questions about genome-wide CRISPR/Cas9...Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting ...

... programmed edit within the editing window (bases 4-8 of the protospacer). While this is a small window... 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library...

...protein, not just fluorescent proteins. Figure 4: Reporters in Gene On include a mScarlet reporter,...for base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using... Additional resources on the Addgene blog Read about other CRISPR reporters Browse our CRISPR blog posts...

...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...bind the RNA motif will be biotinylated. Read more about RapID in this blog! Find plasmids for RapID EXCELL..., S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller...

...Run on a gel and purify the digested vector. Step 4: Ligation Ligate the annealed oligos into the digested...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

...provide longer read lengths of 10 kb to 100 kb (or 4 Mb in ultra-long read mode), enough to sequence an...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

...PMID: 22035192. PubMed Central PMCID: PMC3216358. 4. Lock, Martin, et al. "Characterization of a recombinant... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

...of GVs purified from MDA-MB-231 cell lysate after 4-days of expression (bottom, scalebar = 0.5µm). (d)...you enjoy playing with our genes. Have fun!    About the authors: Marjorie Buss is a PhD student studying...

...enables science: “humility in the face of evidence” (4). Frustration among scientists has been rising as ... with a testable hypothesis - an educated guess about how some part of the world functions. Experiments...governments exist in reality and science is how we learn about reality. Formally, a government can be defined ... More concretely, scientists gather information about reality informing people and their governments on...people to rally for science, others are concerned about possible unintended consequences of a march. Three...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-Science... have more weight than how any one person feels about it. And yet research shows that thoughts on some...

...Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, Gootenberg JS, Abudayyeh OO, ... the Addgene blog Read more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide Browse our entire COVID...

...Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, ...Addgene Blog Learn about using toehold switches to detect Zika virus   Learn more about targeting RNA with...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...with Cas13a (C2c2) Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources...