...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific...

...suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et ...27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker...26422227 Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa...nrmicro3569. PubMed PMID: 26411297 Resources on the Addgene Blog Catch Up On Your Cpf1 Background Read About...piggyBac Vectors in Genome Editing Resources on Addgene.org Check Out Our CRISPR Guide Pages Check Out MpAgo...

...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308. Additional Resources on the Addgene Blog Brush up on Homology Directed Repair and ...for Your Experiment Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages...

... on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody... combines a marker with a signal. For clarity, Addgene uses 'labeling' to refer to antibodies and 'staining...Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect detection of target...Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot Antibodies...

...Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery... Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli...packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S...PubMed PMID: 25716921. Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR ...

...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ...

...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...pAAV-mDlx-GCaMP6f-Fishell-2  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can .... Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one...reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution to expand... coronavirus related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt...

... on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated ... Vectors for Blue White Screening Available at Addgene Are: pUC18 and pUC19 Let’s begin at the beginning...which rescue a lethal phenotype. Resources on the Addgene Blog Read Our Post on Common Lab E. coli Strains...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...

... excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in your own ...details on the production process, check out the Addgene AAV Production protocol. Titering Once you’ve produced...details on the qPCR titering process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested...interested in the ddPCR titering process, which is what Addgene uses, check out our ddPCR for AAV quantitation ...10.1089/1043034041361262 More resources on the Addgene blog Parts of the AAV Packaging PlasmidParts of...PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR Green TechnologyAAV...

...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard. However,...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...PMCID: PMC5167268. Additional resources on the Addgene blog Read more about polymerase chain reaction... vector related protocols Learn more about the Addgene viral service ...of our viral vectors blog posts Resources on Addgene.org Learn our protocol for AAV titration by qPCR...

... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression...deposited their own Gateway-compatible plasmids with Addgene. These can be used to express genes in a variety..., MA: Cell Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

...design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned...lentiviral packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles ...plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR v2,... terms of research impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral...PubMed PMID: 19847206. Additional Resources on the Addgene Blog Your Lentiviral Plasmid FAQs Answered Learn...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus...

...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...resources by sharing plasmids Find plasmids at Addgene ...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...

...have already been built and are available on the Addgene catalog – so check there before building one from...therapy—viruses could further your efforts. And Addgene is here to help! We offer a variety of viral vectors... Click Here to Find Viral Vector Resources at Addgene! References and Resources References Travieso...s41392-021-00487-6 Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression...Editing to Primary Cells Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...

... More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide   ...transduction. Most transfer plasmids available from Addgene were derived from MoMLV (Moloney Murine Leukemia...im1017cs31. PMID: 18432682. More resources on the Addgene blog Viral Vector Elements The Retroviral Life ...This post was written and updated by multiple Addgenies. It was originally published in 2018, in the Viral...

...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies...

...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors...discussed in Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine...