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We narrowed to 906 results for: addgene

Showing: 301 - 320 of 906 results

  1. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...Addgene is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how...offer. Happy CRISPR-ing! More resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other...

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  3. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  4. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage...

  5. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ... perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do...Preparation of electrocompetent cells (protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit...large number of transformations being performed at Addgene, this adds up to a significant time savings. You...

  6. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with ...has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar with the role...I discussed The State of Science with the five Addgene colleagues who read it with me, the consistent ...about many different things (part of the reason Addgene has been such a great fit for me!), the book was...Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein...

  7. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et ...27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker...26422227 Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa...nrmicro3569. PubMed PMID: 26411297 Resources on the Addgene Blog Catch Up On Your Cpf1 Background Read About...piggyBac Vectors in Genome Editing Resources on Addgene.org Check Out Our CRISPR Guide Pages Check Out MpAgo...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308. Additional Resources on the Addgene Blog Brush up on Homology Directed Repair and ...for Your Experiment Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages...

  10. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody... combines a marker with a signal. For clarity, Addgene uses 'labeling' to refer to antibodies and 'staining...Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect detection of target...Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot Antibodies...

  11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery... Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli...packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S...PubMed PMID: 25716921. Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR ...

  12. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...pAAV-mDlx-GCaMP6f-Fishell-2  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can .... Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one...reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution to expand... coronavirus related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt...

  14. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ...

  15. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ... on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated ... Vectors for Blue White Screening Available at Addgene Are: pUC18 and pUC19 Let’s begin at the beginning...which rescue a lethal phenotype. Resources on the Addgene Blog Read Our Post on Common Lab E. coli Strains...

  16. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...

  17. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  18. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in your own ...details on the production process, check out the Addgene AAV Production protocol. Titering Once you’ve produced...details on the qPCR titering process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested...interested in the ddPCR titering process, which is what Addgene uses, check out our ddPCR for AAV quantitation ...10.1089/1043034041361262 More resources on the Addgene blog Parts of the AAV Packaging PlasmidParts of...PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR Green TechnologyAAV...

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression...deposited their own Gateway-compatible plasmids with Addgene. These can be used to express genes in a variety..., MA: Cell Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

  20. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard. However,...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...PMCID: PMC5167268. Additional resources on the Addgene blog Read more about polymerase chain reaction... vector related protocols Learn more about the Addgene viral service ...of our viral vectors blog posts Resources on Addgene.org Learn our protocol for AAV titration by qPCR...
Showing: 301 - 320 of 906 results