We narrowed to 319 results for: bre

1. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different applications that...

2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...is very efficient at introducing double stranded breaks (DSBs) into genomic DNA in many cell types and ...

3. March for Science

   Type

   Blog Post

   Published

   April 21, 2017, 2:30 p.m.

   ...cuts (3, 12, 13, 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern...

4. Evolution of Lab Techniques

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   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...chain-terminating or dideoxy sequencing, was a major breakthrough for determining the order of nucleotides in ...

5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...the basics of Gibson assembly as a parody of “Breakfast at Tiffany’s.” Overview of Gibson assembly The...

6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...distinct cell populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes...

7. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   Published

   June 1, 2023, 1:15 p.m.

   ...., Hu, H., Lee, Y., D’Azzo, A., Messing, A., & Brenner, M. (2004). Expression specificity of GFAP transgenes...

8. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...incredibly useful technique simply by following the abbreviated methods section found within a published manuscript...

9. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ...resistance gene makes stable cell line creation a breeze. Modular by design: Key elements (promoter, tag...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish...

11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy:...

12. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.   ...

13. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...was hard; I was researching the epidemiology of breast cancer and I loved the practical aspects of testing...

14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ... IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as well as teaching and mentoring...

15. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...terminal repeats (LTRs), induces a double-stranded break, and excises the transposon from the donor DNA. ...

16. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...basket. There are many factors that can make or break a lab experience. Previous Addgene blogs have discussed...

17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...talk about it. DNA ligases are enzymes that seal breaks in DNA by joining 5 ́-phosphorylated DNA termini...

18. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ... find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol), which help...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...PMCID: PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon imaging...