.... “When PhD students graduate there has to be champagne, that's a given.” Furthermore, anyone leaving ...fellowships, jobs, or paper submissions with dinner and champagne in the lab. The Church Lab celebrates successful...toasts a newly minted PhD with food, cake, and champagne. What does your lab do for fun as a group? The...those have been trips to breweries who happily exchange our expertise with lots of free beer.” He also...

...application process can dramatically increase the chances of being seriously considered for an open position...steps to submitting a resume that will give you a chance at an interview:  1) Have an excellent resume ready...or location. The more people you meet the better chance you have of finding a beneficial connection. LinkedIn...resume forwarded personally gives you a much better chance at an interview –but you still need to nail the...

...covalent bonds, so there were no weak links in the chain.  Addgene: What about the Streptococcus pyogenes...antibody tentacles may have an impact on this challenge either for magnetic capture or for capture using...this tool? Howarth: Polyproteins are useful for mechanical study of proteins by atomic force microscopy ...particular tips or tricks for those wishing to purchase and use the SpyLigase system for the first time...

...are ever recycled, so adopting a few meaningful changes in the lab could make a big difference in our global...Many scientists are already leading the cultural change toward responsibility in our lab’s environmental...Labconscious. If you’re inspired by this post then I challenge you to take to action! These days, it’s important...’s up to all of us to work on this issue. Be a champion for this important cause! To get more lab sustainability...

... to students.” Making science approachable is often the most challenging part of the job, but Caitlin ...lab classes. However, their opinions are often changed after seeing a kit for themselves. “You open up...Center. And make sure to subscribe to the Career Channel of the blog so you don’t miss the future posts ...

...appropriate antibiotic. Although the precise mechanistic details of the process are not well understood...resultant products of the reaction in the figure from Chaokun et al., 2011 to the right. After PCR, you should...with any PCR-based cloning technique there is a chance of introducing an unwanted mutation. Tips for high...(2016): 145-154. PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free...

...40 cas gene families have been characterized. To date, 83% of archaeal and 45% of bacterial genomes utilize...defense against invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The...Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, ...

...difficult time. One function that rose to the challenge was our Finance Team. With Addgene closed for ...financial crisis. As a nonprofit organization, Addgene charges enough for our services to run our operations. ...to this need, we're incredibly grateful that the Chan Zuckerberg Initiative was able to step in to be ...laboratories all around the world." We thank the Chan Zuckerberg Initiative for supporting Addgene by ...

...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...secondary antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Antibodies 101: Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene....

.... CRISPR 101: Homology Directed Repair Addgenie Chari Cortez explains some of the basic science behind...contributors David Wyatt and Dale Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind...Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check whether you’ve...for your next experiment? In this post, Addgenie Chari Cortez guides us through some common controls and...

... wanting to study bacterial genetics. This all changed thanks, in part, to a collaboration initiated at...Stanley Falkow, Stanley Cohen, Herbert Boyer, and Charles Brinton concocted a wild idea of using the newly...highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and plasmid tags...Plasmids that Glow" and "Viral Expression Vectors" chapters, and more pictures and pro tips.  Happy reading...

...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence..., and thus quantify, due to small fluorescence changes of the donor and acceptor after proteolytic cleavage...fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... cell heterogeneity, and/or cell position changes (To et al., 2016, Zhang et al., 2019). Thus to effectively...

...flexible work schedule the more we realize how casual chatter is very much missed in the virtual world. I believe...audience into small sessions and do a quick break to chat. This can be done randomly or manually assigning...could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas. Social no-science-y...presentation will appear here, and only general chatter will be allowed with a different host each time...

...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...information they’ve absorbed if they don’t get a chance apply it in an interactive setting. The problem...could be played when and, depending upon who your challenger was, you might have to drastically alter your...please freely share your projects with us! This exchange of ideas will help improve life science and biomedical...

...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...attach important references that serve to further characterize a protein of interest (and we very much appreciate...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ... plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection ...

..., the books center around a cast of endearing characters who decide to take matters into their own hands...the 1980s, Sarum follows an intriguing cast of characters from five different families over several millennia...one. On a purely selfish level, I wanted to be challenged to read some books outside of my comfort zone...s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate degree...

...Skip to Chapter 3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice...the influence from his two PhD advisers who were “chalk-and-cheese”. While one was an established Professor....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

...overall positive charge on DNA molecules so that it can more easily cross the negatively charged cell membrane...role in bacterial evolution and is the primary mechanism by which bacteria have gained antibiotic resistance...electroporation, the application of an electrical charge to cells, to increase cell membrane permeability...

... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3 domain podcast segment...biochemical processes. One of the largest and best characterized of these is the Src homology 3 (SH3) domain ...

...available in your machine before you make your purchase. Picking a single fluorophore for an experiment...overlap, or the signal "spilling over" into another channel's detection range. Though you can compensate for... University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core. Retrieved January ...flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf Syracuse Flow Core. Syracuse flow core spectral...