We narrowed to 372 results for: ena

1. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...interest at high levels. Unlike vectors that express screenable or selectable markers from a unique promoter,...

2. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you...

3. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ...come to you (a TikTok scicomm account aimed at octogenarians is going to be a hard sell!) Digital platforms...

4. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...directed localization of the fluorophore, and thus enabled labeling of particular structures (Table 1). Each...

5. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by cutting the vector with...

6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base...

7. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet to be fully realized...

9. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...making pretty pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists...

11. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...two halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...-toxic and could be expressed in living cells, enabling the study of dynamic, physiological processes....

13. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...home no matter how busy I am. Believe it or not, teenagers just go to bed and you’ll never know it so don...

14. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...quantitative, NGS-quality analysis of CRISPR editing - enabling a ~100-fold reduction in cost relative to NGS-...

16. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...specific attachment sites via sequence engineering, enabling even more efficient integration activity. They...

17. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ...glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous...

18. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...classroom, in their lab, in their experiments, to enable virtual learning during the pandemic. Feedback ...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...using/depositing. Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene...

20. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...PE7, it will be exciting to see future studies enabled by this tool as well as additional potential improvements...