We narrowed to 322 results for: nans
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TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is...
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Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences." The... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....Protein Expression Kit has been used to produced recombinant proteins for in-vitro assays: Koch, Daniel, ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m..... Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis ... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....administered systemically, without causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target effects are nearly impossible... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....or when the gene is constitutively-active or a dominant-negative mutant. Reducing the copy number can ... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....American system), in 2009 (at the beginning of the financial recession) I knew obtaining work in scientific... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....: managerial duties ex: order materials, lab maintenance Administrative assistant: assists PI with admin... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become ... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....cells are growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....Andy Fire’s lab used oligo-mediated knock-in of a dominant mutation, known as co-conversion (Arribere et ... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....writing for the Journal of Science Policy and Governance. Get involved! The best way to learn about science... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m.... happens due to a process called Fluorescence Resonance Energy Transfer (FRET). Figure 1: Emission... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m.... the annealed cr:tracrRNA is then mixed with recombinant Cas9 protein to form the ribonucleoprotein complex... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive and time-consuming... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....Rajakumar, P. D., Prekop, H.-T., Chew, Y. T., Manivannan, S. N., & Chew, W. L. (2021). Programmable C:... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....management, alliance management, business development, finance, and operations. To learn more, visit www.propelcareers.com...