We narrowed to 363 results for: nsf

1. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to reflect the workforce." Science ...

2. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...mixing with the liver paste, these E. coli are transformed with the appropriate plasmid and dsRNA expression...

3. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...and electronic companion technologies that has transformed the assay’s capabilities. Specifically this includes...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated ...

5. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...lentiviral CRISPR library that targets human glycosyltransferase genes and other proteins related to cellular...

6. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...

7. How to Prepare for an Industry Interview

   Type

   Blog Post

   Published

   July 9, 2020, 1:15 p.m.

   ... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...

8. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

9. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system...

10. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite sequencing. ...

11. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...cell surface. When the cells interact, GFP is transferred to the receiver cell, and the receiver cell turns...

12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...

13. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...to think about their career options, gaining transferable skills such as leadership skills can only assist...

14. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...tag. The two PCR reactions are then mixed and transfected along with a Cas9 expression vector. They can...

15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking intracellular...

16. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...loci within a single cell. One might imagine transfecting cells with both the nuclease-positive and nuclease-null...

17. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...

18. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...too young and should not be used for important transfection experiments right after they’ve been thawed....

19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ... alter the ability of one FP to nonradiatively transfer energy to the second FP, often decreasing the ...

20. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...