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Showing: 301 - 320 of 597 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture...proteins at endogenous loci, shedding light on previously unknown protein functions. References Schwartz...

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...These benefits of Cas3 do come at a cost — the previously mentioned issues with expressing all Cascade ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/...

  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic control of biological systems...exist at all. Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...

  10. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The... less. The list goes on... In this post, we’ve reviewed many of the most popular cloning methods out there...

  11. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras...different mutant versions of Ras that are most prevalent in human cancers, and synthesizing compounds to...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... lab, or by a previous group. If the plasmid was made by a modifying a vector previously deposited with...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...large field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features...

  15. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are also...cautious during the aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... vector can still occur even in the case of no previous immunity existing for that adenoviral vector (...(2019). Are adenoviruses zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679...

  17. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...more accessible way to image biological features previously only visible via techniques like super-resolution... disciplines now available. In this post, we’ll review the inspiration behind ExM, how it works, and some...

  18. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...large number of honorees to have trained under a previous laureate (Zuckerman, 1977; Ma et al., 2020). Many... to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman in ...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity and accuracy...recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation...
Showing: 301 - 320 of 597 results