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We narrowed to 438 results for: sequence

Showing: 301 - 320 of 438 results
  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...
  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ... still able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter...downstream of a promoter containing the target sequence of the DBD. The DBD-POI fusion (commonly referred...
  3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...However, iChIP requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA...to suppress PCR amplification of specific DNA sequences. Thus, we've made some contributions in technology...
  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu lab also used natural and...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...evolution efforts yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly...editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication ...
  5. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...for your research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed ...
  6. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...science and more! All Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice...
  7. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged DNA and...
  8. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website...
  9. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...the University of California, Santa Cruz, offers sequence data on five strains of Ebola representing the...
  10. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged analysis...classification system relies on a combination of sequence similarity, phylogenetic analysis, neighborhood...that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms of...
  11. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... replication problem. Iterons are repeated sequences within the origin of replication and are required...how compatible their plasmids will be based on sequence data. However, if these do not provide clarity...
  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...unable to methylate cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression...-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ...
  13. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...services: We continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production...establish.These funding decisions will have far-reaching consequences throughout the scientific community for years...
  14. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...even be able to alter the type of nucleic acid sequences that Cas9 can target." As further proof of just...
Showing: 301 - 320 of 438 results