We narrowed to 430 results for: sequence
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TypeBlog PostPublishedFeb. 13, 2014, 1 p.m.... 3-6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene Tip: Often researchers will ...
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CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....than BE3, but APOBEC3G displayed less predictable sequence preferences. The Liu lab also used natural and...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...evolution efforts yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly...editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication ... -
AAV Packaged on Request is Here!
TypeBlog PostPublishedJan. 10, 2025, 1:01 p.m....testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged DNA and... -
Editor's Choice, October 2016
TypeBlog PostPublishedNov. 4, 2016, 2:30 p.m....science and more! All Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....for your research. Improved nucleic acid sequence detection Feng Zhang’s lab recently developed ... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....the University of California, Santa Cruz, offers sequence data on five strains of Ebola representing the... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....and archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged analysis...classification system relies on a combination of sequence similarity, phylogenetic analysis, neighborhood...that are in the same class and type don’t share sequence similarity but are grouped due to mechanisms of... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m.... replication problem. Iterons are repeated sequences within the origin of replication and are required...how compatible their plasmids will be based on sequence data. However, if these do not provide clarity... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....discussions in my lab about whether or not to keep DNA sequencing singletons (reads that only appear once) in our...analysis pipelines for DNA that group similar sequences together in bins, we thought that binning Singletons... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....the EBNA-1 sequence that encodes for the DNA binding protein EBNA-1 [7]. These two sequences are necessary... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....unable to methylate cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression...-deficient; best for plasmids with repetative sequences. F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ... -
Addgene Stands with the Scientific Community
TypeBlog PostPublishedApril 23, 2025, 1:30 p.m....services: We continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production...establish.These funding decisions will have far-reaching consequences throughout the scientific community for years... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog PostPublishedJune 16, 2015, 2:15 p.m.... 6 Tips for Analyzing and Troubleshooting DNA Sequencing Results ICYMI: Addgene’s Plasmids 101 eBook Protocols... -
500,000 Plasmids Shipped and Counting!
TypeBlog PostPublishedMarch 26, 2015, 3:24 p.m....Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....even be able to alter the type of nucleic acid sequences that Cas9 can target." As further proof of just... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known motif serves... of the 11 IPI antibodies contain RGD-mimetic sequences that imitate this motif and compete with both ... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....phosphoserine incorporation Extensive genomic sequencing of prokaryotic lineages revealed a curious anomaly...translation in response to the presence of TAG sequences in ORFs. In this bacterial strain, TAG is an ‘... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design. Best wishes ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher’s collaborator... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....control over gene expression that go beyond genomic sequence. Dysregulation of the epigenome (the sum of epigenetic...epigenetic modifier could be targeted to specific DNA sequences. Non-editing CRISPR applications direct catalytically...