Skip to main content
Addgene
Showing: 301 - 320 of 422 results

  1. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... replication problem. Iterons are repeated sequences within the origin of replication and are required...how compatible their plasmids will be based on sequence data. However, if these do not provide clarity...

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...unable to methylate cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression...-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ...

  4. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...services: We continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production...establish.These funding decisions will have far-reaching consequences throughout the scientific community for years...

  6. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage our website...

  7. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...even be able to alter the type of nucleic acid sequences that Cas9 can target." As further proof of just...

  8. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands. This well-known motif serves... of the 11 IPI antibodies contain RGD-mimetic sequences that imitate this motif and compete with both ...

  9. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...phosphoserine incorporation Extensive genomic sequencing of prokaryotic lineages revealed a curious anomaly...translation in response to the presence of TAG sequences in ORFs. In this bacterial strain, TAG is an ‘...

  11. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design. Best wishes ...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...control over gene expression that go beyond genomic sequence. Dysregulation of the epigenome (the sum of epigenetic...epigenetic modifier could be targeted to specific DNA sequences. Non-editing CRISPR applications direct catalytically...

  15. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...expression, Tanenbaum et al. modified the GCN4 sequence to increase its alpha-helical structure and stability...

  20. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... (2016). Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in ... J., & Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven...to read your experimental output; and (4) the consequences to your experiment if there’s off-target expression...
Showing: 301 - 320 of 422 results