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We narrowed to 710 results for: tat

Showing: 301 - 320 of 710 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...techniques offer researchers different advantages and limitations.  The four main factors in which chemogenetics... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to... once can result in several hours of neuronal excitation or inhibition. This sustained activation period...

  2. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...balance and were content. As a worker, there are expectations whether or not you work in the lab. My first...capstone projects during the Summer Intern Poster Presentation.  As someone who had limited experience ..., I worked on my capstone project report and presentation, during the final week at Addgene. This report...

  3. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. ...fusion protein while it is still bound to the glutathione resin, eliminating the need for an extra clean...

  4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... making plasmid maps, but can also analyze and annotate any DNA sequence. SnapGene Viewer: The free SnapGene...donation-based plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained...analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ... the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2...resulting in a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio...These plasmids are available as a collection, facilitating screening across the transducerome, to gain ...

  6. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...another email notifying me of my acceptance. I was ecstatic! I was going to become a serious scientific wordsmith...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique... D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression in Pseudomonas...

  7. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...research is improving lives worldwide and the presentation made it clear how important it is for us to ...say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles! We also...early on Saturday morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the ...

  8. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...wild-type version of YGOI to rescue a mutated strain or a mutated version of YGOI to test the different...different phenotypes of the specific mutations. For these purposes Addgene has a large collection of yeast expression...

  9. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...negative regulation and can be manipulated with mutations in the replicon. For example, the pMB1 ori maintains...per cell, while pUC – which differs by only two mutations – will produce as many as 700 copies per cell.... numbers since the bacteria have just reached stationary phase, but the cells have not started to die ...

  10. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...calls, how about trying your hand at a video presentation? Most video platforms make it easy to record...get their input as well. Giving a great video presentation is going to be a useful skill for the foreseeable... Science: Where to Search for Funding The Ohio State University; Graduate Student Funding Opportunities...

  12. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...your messaging and your expectations of your audience, and their expectations of you, consistent and clear... racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media consumed...

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on a reduced glutathione matrix...protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis...

  15. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ... Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in vivo Nathaniel...received by a cell. These so-called recombinase-based state machines could potentially be used to report upon...this post to learn more about recombinase-based state machines and how they function. Adapting Toehold...

  16. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...journal will help a Mentee track progress, adjust expectations, and record milestones in reaching goals. The...that I try to exercise every morning, but the temptation to skip the gym is strong. There are a few women...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

  17. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...Now with the DAP array, it is possible to use the state-of-art base editor, prime editor, or any CRISPR-...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...

  18. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...  The iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared...possible to do things like in vivo imaging.   Excitation with NIR light also produces less non-specific... non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be ...

  20. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...Computer and writing skills Writing a thesis or dissertation demonstrates that you have advanced writing ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...
Showing: 301 - 320 of 710 results