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We narrowed to 694 results for: ust

Showing: 301 - 320 of 694 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots of bright...response, brightness, and photostability. It enables sustained deep-tissue imaging in vivo. Image reproduced ...pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE   Liu Z, et al. Sustained deep-tissue voltage recording using a fast indicator...

  2. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...sensitive to single base pair changes, but the edit must occur at an existing restriction enzyme recognition...and small edits (Brinkman, et al., NAR). It works just like TIDE (outlined above), except that it uses ...edits in individual clones. The drawback is that you must generate a DNA molecule with homology to your genome...

  3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ... Gibson assembly, long primers for each fragment must be designed and ordered, and are specific for each... exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or absence of dNTPs...enables one-step targeted cloning of large gene clusters. Nat Commun. 2015; Sep 1;6:8101. PubMed PMID: ...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...or protein expression. If you were to include customized strains, the number is probably in the thousands... with a minus sign (-) as is typically standard; just having the gene listed indicates it is non-functional...strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse ES...PMC4525291 Quadros, Rolen M., et al. “Easi-CRISPR: a robust method for one-step generation of mice carrying...PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline genome engineering ...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...choosing an AAV are… location, location, location! Just like this common real estate mantra, the choice ...2013) What does this mean to us? Well, an AAV user must determine which serotype is most efficient at transducing...proteins associated with these complexes are so robustly expressed that anatomical assessments can be easily...

  7. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... is an important feature for your experiment; it must be chosen carefully. Often researchers want a simple... and Transduction Protocols   Once purified, you must titrate your viral particles to determine their ...protocols and plasmids.  References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus...

  8. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...keep increasing, as efforts using large-scale clustering and discovery algorithms find new candidates ...of rare CRISPR-Cas systems with deep terascale clustering. Science, 382(6673). https://doi.org/10.1126/...s13578-018-0255-x Taylor, H. N., Laderman, E., Armbrust, M., Hallmark, T., Keiser, D., Bondy-Denomy, J...

  9. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Polyclonal antibodies have the advantage of more robustly recognizing the target protein because the antibodies...saving time and money. Nanobodies and synbodies are just as specific for their target antigen as canonical...Assay). Immunoprecipitation Sometimes, instead of just detecting the presence of protein within a sample...

  10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...for the donor and acceptor fluorophores. The donor must be delivered to an exact spot (a single mailbox ...[Yan & Bruchez, 2015]). To get you started, two robust orthogonal labeling strategies are presented (Fig...acceptor-binding site on your protein of interest: you just don’t know it yet! The poly-histidine tag often ...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...improve cutting efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within...bound by the phosphate backbone. These PAM bases must be present for Cas9 to unwind the DNA and check ... et al., 2018). Each matching DNA:RNA pair gives just enough stability for the next DNA base pair to be...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene regulatory...concern, using a purification method that removes, not just reduces, empty viral capsids in your prep can help...Chen et al., 2023). And these differences occur not just between mice and NHPs; some capsids show both different...2019). Selection of an Efficient AAV Vector for Robust CNS Transgene Expression. Molecular Therapy - Methods...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...written by Mary Gearing in August 2016 and was updated by Jennifer Tsang in August 2020 and by Emily P. Bentley..., their other experiments suggest that ABEs are robust and specific editors. Improving adenine base editing...

  16. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...processes, don’t hesitate to contact our friendly customer support team at [email protected].  Curious about...

  17. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...week - be sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching a... many of these resources that we created filters just for them on our search page. Use the Expression ...

  18. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...has officially welcomed the Christmas spirit. It's just unfortunate that we had no choice but to place it...safety regulations. That’s one sticky situation our customer service can’t get you out of.           View...

  19. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...design. In case you need more convincing, I’ve got just the proof: Above our office team shows their love...year. They showed us their dedication to great customer support by dressing up as our Zendesk email system...

  20. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...the research community. Customers have taken advantage of the customizability, choosing the cargo, serotype...
Showing: 301 - 320 of 694 results