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..., 2020, 2022). I do mean highly multiplexed: you can image over 65 different parameters using this method... from Radtke et al., 2020). This flexible system can be used with many conventional microscopes, amplified...that they used in their experiments. Researchers can then visit the IBEX imaging community discussion ...conversations with other users. The entire dataset can be downloaded from Zenodo, including the reagent ...for easy filtering and sorting. Reference images can be viewed by clicking on the links in the "Agree"...detergent, antigen retrieval conditions, and more. Users can also recommend or not recommend a particular reagent...discussion forum where scientists from around the world can ask and answer questions. Andrea and Ziv were...

... reach. We can’t save you the hard work of reading papers and doing experiments but we can definitely ...large section of open access life sciences data. You can use ReFigure to coherently save analyses with the...insights on a topic. Beyond collating other’s data, you can even include your own data in a ReFigure. For example...Figshare is currently supported by ReFigure beta) you can include it in a ReFigure. ReFigures are good for ...proposals. ReFigures are one such research product which can convince reviewers of your expertise and insight....minutes, visit our YouTube Channel. Currently we can only connect figures from PubMed Central, eLife, ...Download ReFigure extension and visit ReFigure.org. You can reach the ReFigure team at [email protected] ...

...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a 20-step...this is why we have a dedicated support team that can help you through any search, ordering, technical,...adding self service to the repertoire of tools you can use to get support for our resources with our new...asked questions. On the Help Center webpage, you can browse through our various FAQ categories or search...enter your name and email address, after which you can describe your issue and press send. Doing so will...directly to our support team’s attention, where we can then offer the same high level of direct service ...from us.   Try out the Help Center! The Help Center can be found at help.addgene.org, and the widget is on...

...similar interests and goals. The following questions can serve as a guide when selecting a research project...What are possible pitfalls in your strategy and how can you mitigate them? If you were to write a grant for...skills do you need to use to reach these milestones? Can you get assistance from people who have the required...What is your timeline? A typical research project can take several years to complete and has multiple benchmarks... new tools and methods that have been developed. Can you think of a project that combines an exciting ...own subject - maybe something used in astrophysics can be applied to fungal biology.” Connie Cepko advises...complexity of biology and break apart a piece that can address a topic in a mechanistic way using the technology...

...before their paper can be published. To a scientist, all of this checking and formatting can feel like yet ...lot of time. It doesn’t have to be this way. We can never completely eliminate the risk that a minor ...stability in an incubator), but there’s a lot you can do to help other scientists both what you did and...scientific citizen” and making sure you do what you can to help your fellow researchers advance science overall... Communication is the issue Much of the problem can be attributed to communication, and in particular...Admittedly, the differences are often very subtle, and it can take a lot of detective work to figure them out. ...accessible and consistent way so other researchers can use them, to making sure that methods and protocols...

... with guidance and training so that you yourself can become an expert and bring the knowledge back to ... perspectives. For trainee level researchers, it can expose them to a new institute and mentor that they...experiencing a new city or maybe even a new country can also be exciting! Identifying a host institute or...personally know or your lab collaborates with that can fulfill your research needs, that is probably the...visiting positions within defined labs which you can apply to. These structured types of placements often...visit, if you don’t have a formal connection, you can just reach out to start the conversation. Many researchers...plan with your host prior to arrival so that you can work together to ensure your expectations are reasonable...

...Viruses are the SUVs of molecular biology – they can deliver materials to specific destinations, haul ...research all the way to clinical gene therapy. But how can you use these amazing tools in your experiments? ...cargo is typically their own genome, but this step can be engineered to be anything you want delivered to... to your target cells. This delivered DNA/RNA can then be either permanently integrated into the genome...application, our Viruses as Biological Tools blog can help you learn about the different types of viruses...degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily, long-...turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...

... way, you can run one PCR, one gel, etc. for multiple plasmids at once. Over time, this can save you a...how much time you have to spend cloning before you can start your experiment? What about all the reagents...eternity of cloning, waiting, and repeating before you can finally dive deep into generating the data you’re...Table 1). Restriction enzymes and vector purchases can raise the cost anywhere from a few dollars to over...varies hugely based on a number of factors, but we can assume it’s at least equal to the $155 spent on reagents...short on money or time, there are a few options you can explore to maximize the use of both when making plasmids...) Ask the PI If you want a plasmid for free, you can always reach out to the PI or corresponding author...

... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...single exon) and everything in between those sites can be excised by intentional introduction of the Cre...mRNA transcripts. When transiently introduced, they can deplete endogenous protein levels for 1-3 days. ...your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have many ...expression. There are several flavors of activators which can be used with this approach - read more about them...you! Many of the tools highlighted in this section can be used to turn expression on/off for short or long...tags Tagging relevant proteins with a degron tag can provide inducible protein degradation. These systems...

...condition that can apply enough pressure to select for variants of interest, though. A selection can take the...The process can range from a defined pathway or reaction to a complex event such as cancer or even aging...identify new players in a biological process. Players can be proteins, genes, enzymes, drugs, or anything else...that’s where the differences in these approaches can be found. As an example, say you are considering ..., you are not specifically looking at cells that can survive. Instead, all manipulations are assessed,... drug-containing media in which only cells which can tolerate the drug survive and everything else dies... to look at an outcome. Your scientific question can help determine which is right for you. Screen variations...