We narrowed to 432 results for: sequence

...The amplified library is verified through NGS sequencing to confirm library representation. Transfection...then be used for genetic screening, followed by sequencing and further data analysis. Based on Figure 31.5.2...need to verify your library by next-generation sequencing (NGS). The representation of your amplified library...each library’s page, you can find protocols for sequencing, representative NGS results (read counts or another...whether selecting colonies and performing Sanger sequencing will suffice…and the answer is no. Due to the...

...eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from viruses, but ...leading to separation between the end of the 2A sequence and the next peptide downstream.4 The "cleavage...experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G D V E E N P ...of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (...Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et al. (Hum Gene Ther. 2009 Aug;20(8...

...walkthrough of how you can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie... generate C->T or G->A transitions in a target sequence in live cells. This technique uses catalytically...a deaminase to make the transitions at target sequences specified by gRNAs. Caveats, potential specificity...

...without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays these...these requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the...Adler et al.   You can also search by protein sequence with BLAST, using the homepage search bar. Once... other parameters, such as E-value for protein sequence searches. Additionally, the Phylogeny tool allows...

... as a guide to complementary RNA sequence. Once a compatible sequence is found (typically RNA that the...purposes similar to that of vaccines. If you know the sequence of a virus, you could introduce some of its dsRNA...due to their abundance of R-loops and repetitive sequence. All of the R-loops described above play distinct...productive levels of R-loops and putting DNA at risk. Consequences of duplexed RNA Mammalian cells have multiple...sensing systems fail, diseases and steep cellular consequences arise. dsRNA An immune response is activated...Cellular origins of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). ...

...presence of any specific RNA sequence. In the absence of the target RNA sequence (in this case, norovirus ...strains and even other viruses by modifying the sequence of the toehold switch. For example, Green has ...proteins using peptide-phage display and deep sequencing, revealing that they bind to a much larger subset...

...with a 1.9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More...libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-...backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used...

...Yamagata et al used previously published nanobody sequences that targeted the following antigens: GFP, RFP...actin-binding protein gelsolin, but any nanobody sequence could be used to make a RANbody. RANbodies are...secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture...useful RANbody, consider leaving a link to its sequence in the comments below. References Sanes, J.R....

...whole exome sequencing of embryo derived-ES cells.They also conducted whole genome sequencing in selected...rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing sites in the...off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos. Since...

...easy enough to contemplate DNA in terms of its sequencing, in vivo studies should always take DNA’s chromatin...expression pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts...of auxin, this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged...(minimal 44 amino acids auxin inducible degron sequence) tagged proteins. Degradation of your protein ...