We narrowed to 932 results for: Gene

...knockout screening, sgRNAs guide Cas9 to cut targeted genes, creating mutations that prevent their expression...expression. The knockout cells can be used to identify genes that underlie a particular phenotype. Figure...knockout screens: sgRNAs to guide Cas9 to targeted genes. In these applications, however, nuclease-dead Cas9...contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each ...are currently 390 pooled libraries available at Addgene, with more added to the collection all the time...barcodes help librarians track individual books, genetic barcodes allow researchers to identify specific...technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also includes a base editing sensor...

...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...

...on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search... this form or the hashtag #BlugeneVirusFriend on Twitter. As with Blugene’s name, we’d like to give their...receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's new virus friend here Additional...eight years now has a new friend to join them. Blugene would like you to meet...  Hmmm, they need a name... we need your help in finding a good name for Blugene’s virus friend.  During the month of January,...

...experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid...Campaign. How to suggest a plasmid The scientists at Addgene work hard to seek out plasmids to add to our collections... potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid, the name of the plasmid ...

...and genetics. It can be used to characterize the function of certain regions in a promoter or gene, as...experiments. When REPLACR-mutagenesis goes head-to-head with Gibson assembly and GeneArt cloning, the results...REPLACR-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works ...your hands!     REPLACR-Mutagenesis Megaprimer PCR Gibson Assembly GeneArt Cloning Types of Mutations...Comparison of REPLACR-mutagenesis with other common methods for site directed mutagenesis   References 1. ...al. (2016). “REPLACR-mutagenesis: a one-step method for site-directed mutagenesis by recombineering.” ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular...

... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... it was another successful event! Addgene company culture At Addgene we organically developed the philosophy... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...

... be done with RNAi in terms of determining what genes are involved in biological process X, you can do...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ...use in genetic screens and has contributed much of his knowledge to the CRISPR resources at Addgene. His... is what got him involved with Addgene - he says he’s “known of Addgene basically since the beginning ...ethic that Addgene has behind it is something that very much resonates with me.” About Addgene’s role in...Doench says, Addgene is “a central resource for what is worth having. The work that [Addgene’s] outreach ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors ...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ... Competent Cells” has been extremely popular, generating many additional questions. We’re happy to see...

...of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering Addgene: For those...brain through optogenetics research Addgene: What are some of the big problems that optogenetic tools can ...science.' Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed...populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions...brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a toolset that...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...

...promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators ...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...Versatile and Efficient Cloning System for Plant Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013)...

... Sean C., Genevieve Laperriere, and Hugo Germain. "Droplet Digital PCR versus qPCR for gene expression...vectors and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. PubMed...genomes can limit production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson...virus type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID: 20486768...benchmark for infectivity of recombinant AAV vectors." Gene therapy 17.7 (2010): 872-879. PubMed PMID: 20336156...new viral vector preparations. Addgene has used this method to generally validate our viral vector preparation...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...

... as ‘indels’. While this is convenient for generating gene knockouts, NHEJ repair does not allow introduction...CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms and model systems. We ...were able to introduce the desired mutations in genes associated with dementia, such as APP and PSEN1,...Cas-blocking mutations at both targeted alleles when gene editing.   Figure 1: Techniques to improve...called CORRECT, which, by undergoing two rounds of gene-editing enables scarless introduction of an intended...introduction of specific sequence changes. To generate a specific sequence change the cell must undergo...that desired genome-editing events by HDR can be generated more efficiently by (1) improving editing accuracy...

...sites yourself using PCR, but gene synthesis can save you time. Using gene synthesis, you can request the...If you’re creating a multigene construct, in what position do you want your gene to be in relation to ...to the rest of the genes? And do you want it to be in the same (forward) or in the opposite (reverse) orientation...you a lot of headaches down the line! Cash in on gene synthesis Before you start your cloning, it is advisable... Figure 1: General overview of the MoClo assembly process to produce a multigene construct. Figure...Golden Gate Cloning Additional resources on addgene.org Addgene's MoClo Guide and Kits MoClo Toolkit MoClo...chosen one of the MoClo kits available through Addgene that suits your experimental purposes. But what...

...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...tags for live imaging in C. elegans CRISPR/Cas9 for gene disruption in M. abscessus  Bioluminescent reporter...developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly...Here, the researchers were able to target multiple genes in M. abscessus either simultaneously or stepwise...system is induced to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...collection of epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version...

...scarless cloning. The cloning scheme is as follows: the gene of interest is designed with Type IIS sites (such... allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct...recognition site(s) thus eliminating these from your gene of interest. PCR products are then digested with...ligated following a heat inactivation step. If your genes of interest or destination vector contain multiple...More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...can also be adapted to express multiple gRNAs. Addgene has two Golden Gate-based gRNA assembly methods...

...presence of the protein endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains ...plasmid amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease...nuclease activity. For strains that still carry this gene, there are usually extra steps like heat inactivation...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit...Browse our Plasmids 101 blog posts Resources on Addgene.org Check out the Molecular Biology Reference Find...

... Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse...mutations, the 8-10 month process of conventional gene targeting is lowered to about 2-3 months. But certain...example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest using a 50-80 bp sequence... mouse model generation easi-er! Generating mice before CRISPR The process of generating knock-in mice...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...template. Figure 2: Overview of ivTRT to generate an ssDNA repair template. Although effective...

...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced... to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are...