We narrowed to 511 results for: ATO

1. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...10% expression in primary human T cells and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression...

2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed PMID: 29169465...

3. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of...

4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   Blog Post

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   July 10, 2018, 1:08 p.m.

   ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

6. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ..., and enzymes for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments...

7. Filming Science Videos in the Age of Social Distancing

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   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ... their mask. But while the camera is here, the operator is in a different room.   Design: The previous...

8. Hot Plasmids: Summer 2024

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   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants...

9. Antibodies 101: Beyond Surface Labeling

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   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ...

11. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

12. Site Directed Mutagenesis by PCR

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    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...

13. Evolution of Lab Techniques

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    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...

14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...

15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ... tries 5 separate experiments at once to get confirmatory data for a hypothesis and presents only the ...

16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ... receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin receptor...

17. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated...

18. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...a hose to a nitrogen gas tank with a pressure regulator. Other methods for physical shearing exist as ...

19. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ..., setting up bases and contacts with Spanish laboratories to start working on projects together. List ...

20. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ...to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were engineered...

21. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...it alone. The help of fellow lab members and collaborators will not only get your paper out the door much...

22. Antibodies 101: Validation

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    Blog Post

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    March 24, 2022, 1:15 p.m.

    ...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...

23. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She...

24. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ...Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional...

25. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...Yellow-orange fluorescent proteins like TagRFP, tdTomato, DsRed, the mKate series, or tdKatushka2 (Drobizhev...

26. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ..."Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature methods...

27. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an...

28. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

29. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...

30. Deep Dive: qPCR

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    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ...