We narrowed to 623 results for: SCR

...rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number...

.... “They took a dividing yeast and did a genetic screen to identify the factors that regulate cell division...

... advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas ...

...modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course, ...

...cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector...

...must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can ...

...the downstream steps. For this example, we will describe how to copy a cDNA from one vector into a new ...

... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

...you particularly good at writing and editing manuscripts? Are you interested in scientific policy? Next...

...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent...

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using...

...produce that green fluorescent protein through transcription and translation. Unfortunately, having live ...

...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

...site-saturation mutagenesis, and medium-throughput screening, she increased the affinity of the sensor for ...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...

... LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She...

...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...

...visualizing your data, such as R or Python. Write a manuscript. If you have all the data you need to begin writing...

...Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional...

...relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson...

...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving...

...has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard...

...time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction...

... — for example, the stem-loop structure of transcriptional terminators. Another potential issue is sequence...

...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...

...region of an ITR with a sequence containing transcription factor binding sites can increase transgene ...

.... Physiological factors Unlike the previously described sensors, this class measures either factors as...

...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...