We narrowed to 697 results for: Tec

...Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/...

...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet was...

...to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...

...amounts of protein for any such disease. This technology therefore represents a potential platform that...CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources on Addgene's...

...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

...your plasmid prep We'll discuss the following techniques to increase plasmid yield. Keep in mind that ...that you might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain...

...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

...advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

...the entirety of a cell. That's a big long-term technical challenge. We have a strategy that organizes how... into actionable signals. Let's say I wanted to detect every primary metabolite in a cell. The only way...

...best teachers and mentors pass on more than just technical knowledge, shaping their trainees’ very way of.... The MCC develops and makes freely available technical and pedagogical resources, offers mentoring for...

...knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W...

...CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270-273. PubMed PMID...Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science (2017): eaam9321...

...CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed PMID: 25184501. PubMed... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184. PubMed PMID: 26780180. PubMed...

...them that their findings may be the result of a technical artifact that introduced an ancestry bias into... a reduction in the number of the dependencies detected. Given this new information, Misek notes that ...

...mediates targeted genome editing in plants. Plant Biotechnology Journal, 22(10), 2950–2953. https://doi.org/...through de novo induction of meristems. Nature Biotechnology, 38(1), 84–89. https://doi.org/10.1038/s41587...

...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/...Escherichia coli and Agrobacterium tumefaciens. 3 Biotech, 13(3), 91. https://doi.org/10.1007/s13205-023-...

...Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository continues to expand, we realized... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...

...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609...CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620...

...Sanger sequencing-based analysis tools are unable to detect or analyze complex edits, such as those generated...sample, ICE includes visual representations of all detected edit types in the sample. The visualization helps...

...microscopy of a viral vector solution. Since this technique is intensive and requires an electron microscope...with titrating by qPCR are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013),...

.... Not surprisingly, brain transduction was not detected. They also used an ELISA to measure binding of...widespread gene transfer to the adult brain." Nature biotechnology 34.2 (2016): 204. PubMed PMID: 26829320. PubMed...

...terms of the question, the methods, the tools or techniques that can help resolve the question, the anticipated... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...

...transferable skills. Transferable skills are the non-technical skills you pick up wherever you work, and you’... communicating. After years of focusing on my technical skills with cloning and lentivirus production,...

...the presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid...Danino, Tal, et al. "Programmable probiotics for detection of cancer in urine." Science translational medicine...

... by directly fusing the cargo to dCas9.  This technique has been exploited to localize factors such as...editors, transposases and prime editors. Nat. Biotechnol. 2020 387 38, 824–844 (2020). Moreno-Mateos, ...

...addition to certain staff scientists and those in technical roles. Therefore, institutions are responsible... law. However postdocs, staff scientists and technicians in primarily bench-science positions are not....

... a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your target is inserted...resulting in the expression of EGFP. Using this technique, you can assess several sgRNA’s at the same time...

...dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population of dark...Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned genes: deletion ...

...commonly employed methods for titering IDLVs include detecting vector particle components or performing quantitative...CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt...