We narrowed to 890 results for: IND

...various research applications, they have become indispensable tools for manipulating cells and organisms. ... to cells for genome editing, and introducing inducible systems into cells, among many other uses. Lentiviral...lentiviral production protocol.  One thing to keep in mind is that titering these IDLVs may require a slightly... (2013). High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature ...

...presenters! Labs and benches! Everything was out the window. So, much like SynBio, it was time to start our... was going to be time consuming and difficult. Finding volunteers who had space to shoot was another hurdle...people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several years, you...distance. Since everyone in these shots (and me behind the camera) would remain masked, it freed us up...

...Resources on Addgene.org Find CRISPR Plasmids for Plants Read Our CRISPR Guide Pages Find Validated gRNAs for... for targeting your favorite plant gene; you can find many plasmids for a wide variety of CRISPR applications...Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool. MMBR 67:16–37 . https...

...researcher is searching for), then the probe will bind to the sample thereby labeling it. The label can...southern blotting are conducted on a sample from an individual without sickle cell anemia, two DNA fragments...sequencing could move from sequencing cores to individual labs and even out into the field. Current versions...Resources on Addgene.org Check out Our Protocol Pages Find Molecular Biology Tools Analyze Your Plasmids by...

...Resources on Addgene.org Find Plasmid Cloning Protocols Find Virus Protocols Find Basic Molecular Biology...technologies We've described Sequence and Ligation Independent Cloning (SLIC) in a previous Plasmids 101 post...

...2015) made this step as simple as heat-shocking to induce the expression of a Cre recombinase contained within...editing efficiencies, obtaining as many as 100 independent GFP knock-ins from a single injected animal.  ...elegans has an AT-rich genome, it can be difficult to find an appropriate NGG PAM site to target. The (T)TTN...visualize specific genomic loci (CRISPR-imaging), or to find the proteins associated with a given genomic locus...

...examples of survival of the fittest. Cancer cells find a way to grow haywire, access and create more vasculature...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted...lack of oxygen in the tumor, activates hypoxia inducing factor (HIF-1), which promotes the production ...response against tumors. Some chemotherapeutic agents induce immunogenic tumor cell deaths that help activate...

...Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...system relies primarily on contrast to identify individual objects and perceive the world around us. Without...reflected. This reflection occurs due to the refractive index mismatch between the glass and the water-like buffer...

...consistent annoyance of walking into a fly lab and finding ourselves accosted by dozens of tiny black dots...This innovation system can help fly labs everywhere find and re-contain any specimens that escape during ...   If you're working in fly research, I hope you find this new technology as helpful as we have!  References...

...graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral vectors, applications...Hub, an open platform for scientists using AAV to find and contribute experimental information, such as...Addgene blog Download our Viral Vectors 101 eBook Find all of our viral vectors blog posts Learn more about...

...scientific research. You’ll meet some of the folks behind the journal and hear from a fantastic team of high...      Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to...enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check out our ...

...the nonprofits section.  The Booth What will you find at Addgene's booth? Well, first, Addgenies! We'll...can be more helpful to our community.  You'll also find tons of information about Addgene's ready-to-use...run a booth? Between two and four! So you'll also find Addgenies out and about, attending sessions, visiting...

... Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology Plasmids Browse our ...'s swift transition from graduate work to an independent position at the Genome Institute of Singapore...

...senescence, autophagy, CRISPR, and so much more (find the full, searchable list here). Tips for using ...producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...Service Learn more about our Viral Production Process Find other Viral Vectors ...

...page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for...Genome Engineering Overview and our CRISPR guide to find out how to use genome editing techniques in your...genome engineering. To make it easier for you to find plasmids for your work, plasmids in the Microbiology...

...repositories to find an antibody for your experiment. Search engines let you easily find and compare available... options — or not know where to start looking to find one. Luckily, there are websites that can help: ...

...PMID: 23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang...18(9) (2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources... Experiments  Resources on the Addgene Website Find Cepko Lab Plasmids at Addgene ...

...tricks for those new to the lab, along with some behind-the-scenes peaks of Addgene’s people, culture, ...our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions...check out our Instagram all summer long to see what kind of shenanigans we get into!  We hope you are as ...

...Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular...

... hopes that these screens will both identify individuals who are infected with HR HPV and therefore at...HPV subtypes with risk and disease severity. Individuals who are shown to be at high risk by these screens...Raised Money for Rare Disease at the Superhero 5k Find New Cancer Resources Learn About Our Grants to Rare...

... open to a different antibody option. Or you may find yourself with only two options, but very little ... that data was included in a publication, you'll find that information in the report as well.    Figure... in Figure 3.   At the end of the report, you’ll find a results section, which will tell you if the antibody...

... how can one little change make a difference? Individual changes to our behavior can lead to cultural ...freezer challenge that your university could join. Find alternatives for some of the harmful chemicals in...other scientists save resources by sharing plasmids Find plasmids at Addgene ...

...online accessibility. It is now about as easy to find a paper in a lesser-known journal as it is in a ...-tier publication. Accessibility is surely also behind the increase in citations for older publications...publications. Ventures like Impact Story seek to find ways to capture scholarly work that exists outside...

...world and help them share their lab experiences, findings, and experiments as articles written for general...Google search. Be careful about hidden connotations behind a word. Non-scientists interpret terms like manipulations...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts Sign up to...

... Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...Nature protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the...

...therefore mCherry will only be expressed in neurons. Indeed, this AAV provides you with a means to specifically...coming months so be sure to check back often! To find more viral vectors available through Addgene, be...Viral Service Page Browse Additional Viral Vectors Find Viral Vector Protocols ...

...resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens when...also redesigned our website to make it easier to find what you need and to bring it in line with current...and reinventing our search experience so you can find exactly what you need, faster. We’re also building...

...available once they have a graduate degree in science. I find it ironic that we do all of our training as scientists... list and take notes if that is helpful. Keep in mind that this is a good time to ask personal questions...having to talk with strangers. Be bold in asking all kinds of people to connect with you and to share their...

... expression vector and expression system. If you find that your protein might not fold well, you should...cited below. pCri plasmids are available either individually or as a complete kit from Addgene. References...Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...

...search through the entire FAQ collection. You’ll find topics ranging from depositing your lab’s materials...the support you need. If for some reason you can’t find the answer that you’re looking for, or you just ...Pages Resources at Addgene Visit the Help Center Find Plasmids for Your Research Become a Member of the...

...10.1073/pnas.1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. ...1479-91. doi: 10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. ...10.1016/j.cell.2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...

...chance you have of finding a beneficial connection. LinkedIn is one great way to find an appropriate liaison...application. 3) Read job descriptions carefully Be open minded and be bold. You don’t have to have all of the ...

...disease progression or onset. These hits can reveal individual genes or sometimes even genetic pathways that...of possibilities greatly increases the odds of finding a quality drug downstream. In some cases, there...generate patient mutations and then test both individual and combinatorial drugs on for effectiveness ...

... commonly through viral delivery by AAV. You can find AAV tools for optogenetics and chemogenetics on ...controlled Unique advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...

...organization what do you think of? Maybe the term brings to mind a social service organization like the Red Cross...organization cannot distribute profits to private individuals through stock or investor pay outs. Addgene has...relationship with our community members. We also find that, as a nonprofit, we attract partnerships with...

...experience this very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments Running...protocols, guidelines, and standards. Bricking science reminded us of our inner children who have no preconceived...to Share Your Science Resources on Addgene.org Find Protocols and Protocol Videos Check out LeGO plasmids...

...(and, of course, find plasmids that will be useful to you.) With these goals in mind, we set out to update... plasmid class covered on that page. To help you find popular plasmids, we’ve also added four new plasmid...

...small circular pieces of DNA that replicate independently from the host's chromosomal DNA. They are mainly... markers for use in other cell types. Primer Binding Site A short single-stranded DNA sequence used ...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is...

... and links provided, in addition to a list of individual screens curated for the publication. Clicking...blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library blog posts Check... guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your research ...

... the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system...adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy the... CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have...