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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE

Showing: 321 - 340 of 610 results

  1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...amplified to add 5′ and 3′ homology regions to the adjacent fragment or vector. 5′ overhangs are generated...higher temperature at which Gibson assembly takes place may limit formation of secondary structures at the...

  2. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...can see from Figure 2 that determining where to place your gate based on the dot plot would be complicated...Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource on addgene....

  3. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a result of the...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...information, however, did not improve at the same pace as the quantity and quality of the information. ...down, and zooming out — with an intuitive user interface.   The team approached the issue from multiple...

  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...outside your organization. The mentor in your own workplace is able to: Help you identify specific skills...can tap for email discussions. It is also a great place to practice your mentoring skills. Online mentoring...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ... et al. (2018). Of course, preprints don’t replace journals and other modes of scientific communication...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

  9. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...entails will help keep you motivated when you’re faced with yet another hurdle in your research journey...will always be room to improve. The next time you face a problem, try to be mindful of how you work through...

  11. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release...intensity-based signaling biosensors to monitor intracellular signaling,” says Hébert. Previous research has...

  12. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic cargo. Typically, when researchers speak...dependent switches control gene expression via switches placed in your cell type of interest. Careful combination...

  14. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...communication, be it in your academic lab or your workplace, is an ongoing process. It is never really done...you try any of these approaches in your lab or workplace, let me know. If you have come upon any other ...

  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...from the drop-down and add an explanation in the adjacent Patent/License Explanation field. Filling ...in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send the...

  16. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...

  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...SARS-CoV-2 virus depends on its spike protein to bind ACE2, the host cellular receptor. To more easily study...SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV. The lab...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...co-founder of www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the...clear that you are staying because it is the best place in the world for your research to rapidly progress...
Showing: 321 - 340 of 610 results