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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 705 results for: ANG

Showing: 321 - 340 of 705 results

  1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...are proteins that detect and output the voltage changes in neural activity when paired with appropriate...optogenetic actuators.  To overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted...

  2. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ... in D.C. for a few years, Susan was ready for a change of scenery. She moved to Seattle and continued ... there there’s always plenty of writing: plain-language stories about Fred Hutch research, pieces for ... scientist or someone who’s affected by climate change or affected by a superbug. I think we solve these...

  3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...plasmids modified from pSC101 with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered... On the other hand, slow growth can also cause changes in plasmid copy number. During the shift from exponential... Jennifer Tsang...

  4. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...can be hard to visualize or perturb these slower-changing bioelectric states in living cells. Since bioelectrical...PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction protein-mediated cues control... Angela Abitua...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody conjugation...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based... choice for ELISAs.  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sure the feature you want to verify is within this range. Read some of our other blog posts for more tips... simplify the process. First, nearly all of our Sanger sequencing is done from glycerol stocks (overnight..., and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make sure to...

  7. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ... loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called... Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the Joung lab at Harvard...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...are for the coaching relationship. Do you want to change jobs? Grow your professional presence? Learn a ...and make direct suggestions for improvement and change. Work with the coach to define how you will know...work with you remotely (by phone, Skype or Google Hangout) but in-person coaching is nice if you can get ...

  9. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... govern expression of genes involved in a broad range of reproductive, developmental, metabolic, and immune... expression and reporter plasmids and the free exchange of these vectors between laboratories. The development...Reading: Chawla, A., Repa, J. J., Evans, R. M.and Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...Virus-specific concerns   In nature, some viruses are more dangerous than others, and the same holds true for types...For many researchers, viruses might be the most dangerous things they work with, and that can understandably...let you work with these valuable tools without endangering yourself or others. Happy transducing! Resources...

  11. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...information briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream...society, and an ability to talk about science in a language that can be understood by those who are not experts...communicate in very technical and jargon-filled language in academic writing and presentations. But for...

  12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...the glycan biosynthetic pathway and have a wide range of clinical manifestations. PMM2-CDG is the most...cause of rare diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids...missense mutant alleles that cause human disease change Arg to another amino acid. Clearly, the development...

  14. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...common and will stand out; however, be careful not to hang your poster too low. If it is pinned to low, some...inserted into larger circles or ovals, that are arranged in some logical pattern across the poster. This...into circular shapes rather than a square or rectangular boxes. Background and color One of the best ...

  15. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...experiment. References 1. Kutner, Robert H., Xian-Yang Zhang, and Jakob Reiser. "Production, concentration... 16836756. PubMed Central PMCID: PMC1534021. 3. Zhang, Bing, et al. "The significance of controlled conditions...

  16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...the only right way, and Indecisives, who keep changing their minds and have trouble making decisions....talking about your emotions, such as frustration or anger, because your discussion will get side-tracked, ...instead of email. As you cannot see a person’s body language or sense their tone of voice in an email, there...

  17. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...transformation amplification protocol is the Chong Zhang E. coli Genome-wide Inhibition Library. DNA and...SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors...transcriptional activation screening, a protocol paper by the Zhang lab, and the Broad Institute’s general pDNA Library...

  18. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution...

  19. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...phenomenon you will likely want to monitor the voltage change across the membrane of cardiac cells. Similarly...binding a Janelia Fluor (JF). The voltage-dependent changes in rhodopsin modulate the degree of quenching of...  References and Resources   References Beck C, Zhang D, Gong Y. Enhanced genetically encoded voltage ...

  20. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified in the...Annealing- The temperature is then dropped to a range in which the forward and reverse primers are stable...Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex PCR Amplify...
Showing: 321 - 340 of 705 results