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We narrowed to 889 results for: ANT;

Showing: 321 - 340 of 889 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...then collects the perfusion liquid for quantitation. The advantage of this method is that it can be performed...dopaminergic neurons in a brain region called the substantia nigra, which is the main source of the body’s...inserted into the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid...performed in a live awake animal by surgical implantation of the probe into a specific brain region. While... sensor. This sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when...when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar...GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor, such that the GFP fluoresces...

  2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant called...internal motto for scientists who contact us and want a guarantee that their experiments will work (yes, this...through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed...curious about these newest features of our website.  I want to offer a huge thanks to all the Addgenies who ... helped get me to this meeting. And of course, I want to thank the organizers of this meeting who have...share their cutting edge science in increasingly important field.  Interested in Addgene's Keystone Poster...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...G418-selected clones. However, there are important disadvantages to the AAV-based approach that must be ...this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression...downstream of a functional promoter (gene traps). The resultant gene targeting system could be used to routinely...non-sequence specific frameshift mutations through aberrant double strand break repair, it has proven more...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele...nucleases In an effort to combine the respective advantages of the AAV and CRISPR-based approaches, several...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ... and powerful high-throughput screen to select variants based on performance metrics specifically for ...vivo (Farahani et al. 2022). The authors take advantage of the SH2 domain of ZAP70 (ZtSH2) which binds...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...BeadScan platform for screening candidate biosensor variants for performance under two-photon fluorescence ...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...tdTomato), they produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...cells more resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice ...researchers: the mouse (Mus musculus). Mice have many advantages as a mammalian model organism for scientists ...nervous system. The fly has also emerged as an important human disease model with potential for therapeutic...in the fly (Pandey and Nichols, 2011). A huge advantage of using the fruit fly is the array of genetic...due to their simplicity, yeast have become an important experimental model in the study of neurological... 1960s, the zebrafish has gradually become an important model organism. They share about 70% of their ...Craig Mello a Nobel Prize in 2006. One of  major advantages of C. elegans is that they are transparent throughout...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...Broccoli, and useful for quantitative studies. Specifically, Corn was developed to quantify RNA transcription...that is generated allows for qualitative and quantitative measurement of processes including RNA movement...DFHBI can only be used for qualitative, but not quantitative, studies. Additionally, Spinach, the first aptamer... In the search for something more stable for quantitative imaging, Ming Hammond’s group created Spinach2...Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized... flow cytometry, the Jaffrey group was able to quantify the effect of mTOR inhibition on the activity ...short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV, were then...

  7. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...from their evolution. Southern blotting and quantitative real-time PCR Southern blotting, named for its...DNA identity, size, and abundance. If a scientist wants to know if an organism has a particular gene mutation...commonly used. In all cases, the result can be quantified by the strength of the signal in comparison to...experimental questions. Real-time PCR detects and quantifies a gene or DNA sequence of interest by recording...compare different DNA sequences to see which is more abundant in a sample. Whereas Southern blotting is labor...of high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput... similar experimental questions (see below). Importantly, although their role has changed, restriction...

  8. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it does...genetic disease in a postnatal mouse model, an important proof of concept for future preclinical and clinical... time, which would prevent the accumulation of unwanted mutations over time. DMD is a good choice for ...does not cause obvious germline modification, an important finding given the controversy associated with ...great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler case than other...and large animal models with mutations in human-relevant exons 45-55, rather than in the traditional exon...Potential off-target effects in muscle, as well as unwanted germline editing, must be rigorously examined ...

  9. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...impractical to catalog every variant of a feature. Instead, we identified common variants, and then crafted a ...principles. For every task, we envision what the user wants to do and make the path to accomplishing their goals...with every possible option, we place the most important controls front and center, and make specialized...sequences. These plasmids contain features such as antibiotic resistance markers and replication origins, but...threshold. This issue is addressed by adding more variants to the database. Another limitation is that by...augment the database with new fluorescent protein variants. The future of DNA feature identification What...to newly sequenced strains through our mismatch tolerant detection algorithm and thereby speed up the annotation...

  10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...16528756. 2. Oh, Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic pressure and...Rather than being released into the cell culture supernatant, the viral particles associate with the cell ...induce hypertonicity display enhanced monoclonal antibody production (Oyaas, 1989; Ozturk and Palsson, 1991...scientists rely on our AAV preparations for, we wanted to be certain that this supplementation strategy...test this approach with different capsids. Most importantly, because our AAV yields are improved we will ...18613082. 3. Oyaas, K., et al. "Hybridoma growth and antibody production under conditions of hyperosmotic stress...osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and bioengineering ...

  11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...going to tell you to pick up the pace Constant worry about grants and renewals What is different about...scientists start out in academia, it is especially important for graduate students and postdocs to get a perspective...interesting topics Having strong non-science skills is important for success Must always justify use of resources...outsource a task someone else who can do better, no grant writing Job changes can happen regularly (every ...learn about the diverse options and how to take advantage of them early is one way to smooth your path to...scientists following diverse career paths is an important first step. Developing mentoring relationships...enjoy the most. Having a better idea of where you want to go will help you focus on the transferable skills...

  12. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down...disrupts the function of YFG. You could compare this mutant fly to one lacking the mutation in the same genetic...from having to create a new fly line every time you want to express YFG in a new tissue type (more details...For example, if you discovered five new genes and wanted to express them within a specific neuronal compartment...eg. LexA/LexAop and Cre/Lox systems) in case you want to express one gene in one cell type and another...process of generating your own fly lines. In the meantime, be sure to browse the publication references ... a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed...

  13. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...a PhD student in his first year. “It was very important to me to be with them in the lab when they started...the lab and to take things slowly! It is more important to carefully select first hires who are well-suited... having projects ambitious enough that everyone wants to recruit rather than repel and hoard.” If you'... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at.... “Being a PI is more than just experiments and grant writing. You have to enjoy the mentoring role.” ...every day and in the pub with us in the evening, wanting to know our latest results as soon as the gels ... build a strong, collaborative team. And take advantage of the many resources available from your institution...

  14. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...This is followed by some perhaps surprising, yet important, factors to consider when seeking your dream lab...the type of science we do fits in with what they want to do. The experiments must fit with their interests...short-term goals and chat about awesome science. Important factors in the search for a lab Studies show that...postdoc. Although the chosen research field is important, the end goal during this time is to be trained...role models and teach you to be the scientist you want to be. For much more on this topic see our blog ...neighbors during the interview process. What if I want a non-academic career path?  If you are considering...

  15. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...should I take to get to work?”; we often make important life decisions without much logic - even choosing... of work and communication before making this important decision. I’ll highlight some of these questions... if it is what is best for you. Some of these important questions will be easy to ask the lab head, and...the mentors you choose are teaching the stuff you want to know and display character traits and skills ...the department? Are they successful at getting grants and funding? Beware of bullying Your mental health...your aspiration? Will you help or at least observe grant writing? Get equipment quotes? Understand administrative... a career at a small, teaching college you might want to consider less expensive, portable areas of research...

  16. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...University of Amsterdam. Before you decide which car you want to buy, it is worthwhile to test-drive a couple ...the best advice is to try a couple of promising variants to check how they perform under your experimental...protein properties need to be scrutinized under relevant conditions. The properties of fluorescent proteins...detected signal and therefore high brightness is an important feature of a fluorophore. The key question is ...indicated by the extinction coefficient (EC) and quantum yield (QY) respectively. The theoretical brightness...However, the theoretical brightness ignores several important experimental conditions that are related to the...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

  17. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...Mentoring Group all participants act as both mentors and mentees. Because the participants are at a similar...make mentoring less mysterious and train the participants in skills useful for future mentoring relationships...step-by-step, how-to guide to get one started. Advantages of the group mentoring format Adding peer advice...at the same time Broadens the network of the participants Can be a lot of fun! Form your own peer mentoring...talk to trainees more about peer mentoring. They want to find mentors to help them develop their skills...Discuss your general areas of interest and what you want to cover during the year. Suggest that each person...perhaps a 3 hour breakfast once a month. All participants must commit to making the meetings a scheduling...

  18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme...effective at wiping out large regions of DNA and has anti-viral application potential as a result. Fast facts...enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is...target sequence Similar to Cas12, it is also significantly smaller in size than traditional Cas9. Another...when engaged by its target sequence. Why would you want to give it a go? The high specificity of Cas14 makes... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...reasons why doing something without a kit has advantages: less plastic waste, less expense, and less of...such as RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work...effectively isolate intact RNA, a fast, strong protein denaturant would be required- something that broke down ...Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did just this (Chirgwin...Chirgwin et al., 1979). They developed a protocol meant for isolating RNA from rat spleens in which they...material is removed via centrifugation and the supernatant is extracted with chloroform as in the above ... results of a downstream application such as quantitative PCR to assess gene expression. There are several...

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...in cases of disease prevention. However, it is important to consider that already-tested CRISPR alternatives...cardiomyopathy and other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find... find those that do not carry a given dominant disease-causing gene, or that carry only one copy of a ...clinical applications could be considered. It is important to consider the question of broader applicability...had few predicted off-target effects, but this advantage likely won’t transfer to all genes we’d like to... large step forward in the CRISPR field, it’s important to remember that the study hasn’t achieved any...
Showing: 321 - 340 of 889 results