We narrowed to 821 results for: ARC

...Palm Springs in late January, brought together researchers studying deaminase enzymes across disciplines... dissecting mutational processes, to medical researchers advancing base and prime editing toward the clinic...rapid, worldwide open access and maximize your research’s impact! This blog post was written by Addgenie...base editing efficiency in ABE8e. Nucleic Acids Research, 52(22):13931-13944. https://doi.org/10.1093/nar...off-target activity induced by prime editors. Genome Research, 35(9), 2064–2075. https://doi.org/10.1101/gr.280164.124...

...Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on educational...students in functional genomics research Learn how the Arabidopsis research center brings plant science to...

...increasingly more cost effective and precise, researchers are now using it to investigate potential therapeutics...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...correct disease hadn’t yet been realized. The researchers chose to explore its potential in mice with a...known as Crygc. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...

...development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found that affect...

... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...overexpression of all human transcription factors, barcoded libraries to trace cell lineage, and so much more...

...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic...

...-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies...https://doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies...

...plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...lentiviral prep here Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns... viral protocols and videos Find AAVs for your research ...

...method. Read on to learn how to use it in your research! The starting point: Ligation-independent cloning...only one type of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless...

...expression of recombinant proteins for industrial or research purposes requires stable host cells (the simplest...chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that...commercial operators, government agencies, active researchers, as well as fellow iGEM teams. We were the proud...more productive and collaborative environment for research.  We’ve also involved ourselves in extensive outreach...

... Optogenetics Plasmids for Use in Neuroscience Research Neuroscience covers many subfields When I first...neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect Alzheimer's...projects using biological tools. In addition to the researcher using sequencing technologies to study microbiome...making more AAVs and educational content to help researchers use these great tools. If you’re just getting...

...Drawing on current questions being asked in MIT’s research labs, BioBuilder inspires students and teachers...malleable curriculum, BioBuilder takes cutting-edge research projects in synthetic biology and transforms them...and watching animations that present a specific research problem and experiment to tackle. Next it’s off...and provides a nationally recognized platform in March to exhibit projects at any stage of completion, ...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...secondary antibodies would save a good deal of it. Researchers are continuously looking for ways to improve ...providing needed flexibility in assay development. Researchers who understand how and why to use secondary antibodies...

...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...commonly, antibodies of the IgG isotype are used for research purposes. Isolating polyclonal antibodies from...antibodies are particularly useful for certain research applications. Many secondary antibodies are polyclonal...Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...

...investigator at the University of Cambridge. His research focuses on protein innovations for therapeutic...Promega Plasmid Collection HaloTag Collection of research tools from Promega, including many HaloTag plasmids...Biological Building Click Biology describes the search for robust, reliable reactions, using only nature...these kinds of Click Biology reactions. For basic research, the stable and selective reactions of these tags...

...things. Now, work from Connie Cepko’s lab allow researchers to activate transcription or Cre recombinase ...activation The project began with a frustration many researchers have experienced: wanting to ask and answer experimental...not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to express a variety of ...and we’re eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A...

...responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences, I ... is a style match for your needs can take some research.  It helps to spend some time thinking about what... Academic Labor Environment from the Future of Research Symposium Resources on the Addgene Website Check...Recent Job Openings Find Plasmids to Expedite Your Research   ...

...circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...which is why MoClo is so attractive! It allows researchers to assemble and test many variables all at once...is postdoc in Peter Schultz’s group at Scripps Research, where he works on genetic code expansion.  References...

...required a one-hour presentation about a prior research project. In total, the interviews lasted from ...prepared with questions to ask. With a quick Google search for “questions to ask an interviewer,” you can ... they will ask you to present about your prior research. This is a chance to highlight your prior accomplishments...deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science...

...removing the wild-type TadA from the complex the researchers developed a second set: The ABE8.x-d version...3.2x higher for positions A3-A4 and A8-A10. The researchers selected 8 of the tested ABE8 constructs (ABE8.8... base editing.  Try ABE8 base editors for your research! Base editing in primary human cells  Cell therapy... of ABE8 for primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells...