...environmentally friendly organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based... a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward a few...received 104 million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not...

...can alter the structure of the protein. In some cases this can lead to changes or a complete loss of protein...lipids including those of the cell membrane. In some cases, this can mask epitopes such that immune complexes...only enter cells with disrupted membranes as is the case with dead or dying cells. DNA binding dyes are not...

...In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...

..., and bidirectional transport for AAV1, -8, -9 (Castle et al., 2014). Differences between AAV serotypes...space along an axon, this does not seem to be the case for either AAV1 or AAV9. It should be noted that...PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur,...

...of CRISPR, Addgene gave to me: RNA editing with Cas13.  On the tenth day of CRISPR, Addgene gave to me...to me: methods for adenoviral delivery of CRISPR/Cas9.  On the twelfth day of CRISPR, Addgene gave to ...

...unsuccessful. Listen to the interview with Crystal Shih Podcast breakdown Intro Chapter 1: Who is Crystal Shih?...the audio engineer and producer for the Addgene Podcast.      Additional Resources from Addgene Find more...

...and a project. Listen to the interview here! Podcast Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...Community.” This post leads readers to our latest podcast all about graduate student life and how establishing...Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of...

...contains and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid...pBACKBONE-hGene Tip: Often researchers will add a lower case letter to the beginning of their insert abbreviation...

...World Health Organization says the number of new cases could rise to 10,000 a week within two months. ...has been fatal in 70 percent of the most recent cases, primarily in West African countries. Even that ...

...the audio engineer and producer for the Addgene Podcast.     Additional Resources from Addgene Learn about... podcast...

...technology of the year in our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of.... So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,000 labs around the world...

...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...

...injection site and choosing a specific serotype, but occasionally you may want to further control where your gene...mouse line that contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter...

...Central (also eLife, PLOS and Figshare). Some use cases include: Cataloging a reagent’s performance as ...published, Zika researcher Christa Osuna collated Zika case studies, making a meta analysis available to the...

...positively affect scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed... helped accelerate scientific discoveries.” Occasionally, a plasmid tool is so influential that it is...

...genes at the site of insertion. In both of these cases, transposons combine the advantages of viruses and...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping...

... the other posts in this series. Listen to our podcast interview with Connie Cepko to learn about her ...leaders rarely give orders (although they must occasionally). Good leaders exert influence in other ways...

... a PI who is a good mentor is important in both cases. As a PhD student you need to learn how to be a ...you pursue any career alternative? Listen to our podcast interview with Harvard Medical School researcher...