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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 321 - 340 of 659 results

  1. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...

  2. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...combinatorial expression of other genetic elements including transcription factors (Cheng et al., 2014) and... with effective gRNAs are publicly available, including those found at Addgene! Figure 1: Strategy...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science...

  5. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...

  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...

  8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical...

  9. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have... field of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, ...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...widespread use of FRET with fluorescent molecules, including fluorescent proteins, has led to the alternative...measuring the change in FRET efficiency, from which conclusions regarding the proximity of the two proteins can...

  12. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

  13. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...destroy. One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as... Other treatments currently in clinical trials include, but are not limited to, ones targeting muscular...

  15. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

  16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ... infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and ...can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...

  18. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...
Showing: 321 - 340 of 659 results