Skip to main content
Addgene
Showing: 321 - 340 of 356 results

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...but fret not! The key to saving time and energy during your in vivo work is to screen you gRNA in vitro...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...cells to be used and STR profiling to be conducted during projects to ensure the results are reliable. With...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about...

  9. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...be analyzed to understand human disease alleles during and after embryogenesis. Addgene has over 600 plasmids...

  13. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... fill in the resulting ssDNA. A single HJ forms during this process and resolves by cleavage of the crossed...

  15. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers should ...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...whole plasmid sequencing of our plasmid samples during our quality control process. Since MiSeq involves...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast culture...
Showing: 321 - 340 of 356 results