We narrowed to 364 results for: DUR
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TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....overlooked. Nanobodies are smaller in size, more durable, and usually much easier to clone, express, and...
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Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog PostPublishedApril 16, 2020, 1 p.m....Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....lab, low copy plasmids may result in low yields during DNA purification. On the other hand, they also ... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m.... of the department] at [name of the university].During my thesis work on [phenomenon X], I read several... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....skewed the representation of your pooled library during the amplification process. It’s far better to verify... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....viral genome). Empty viral capsids can be formed during viral production depending on the experimental... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....are obtained. Or someone may heat an ELISA plate during incubation steps in order to enhance the OD signals... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....but fret not! The key to saving time and energy during your in vivo work is to screen you gRNA in vitro... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....cells to be used and STR profiling to be conducted during projects to ensure the results are reliable. With... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....The first time I heard about FRET during a journal club, my guitarist brain automatically thought about... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m.... the cut site were not efficiently incorporated during HDR. We decided to systematically characterize ... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....researchers today generate vast amounts of digital data during their work. It is already becoming impossible to... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m.... allow cells to become persistently fluorescent during development. Stochastic multicolor labeling systems... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....Warner et al., 2014); its length reduces artefacts during live cell imaging. Bunch of baby spinach, or BoBS... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....be analyzed to understand human disease alleles during and after embryogenesis. Addgene has over 600 plasmids... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....High-level overview of an indirect immunofluorescence procedure with key considerations about each step. Image... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m....parameters — H, W, and A — describing the intensity and duration of the detected signal. Using a practical example... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m.... fill in the resulting ssDNA. A single HJ forms during this process and resolves by cleavage of the crossed...