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Showing: 321 - 340 of 378 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...visualize proteins in vivo by tagging them with fluorescent proteins to study localization or purify proteins...

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After...

  7. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... the central nervous system via cerebral spinal fluid. The injection site is the space in and around the...

  9. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...that the amino acids neighboring the Myc epitope influence 9E10’s affinity for the epitope, a phenomenon ...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...associated, such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can ...

  13. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (...

  17. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...through the cell cycle stages. If cells are overly confluent or don’t have sufficient nutrients in their media...
Showing: 321 - 340 of 378 results