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  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...on single-cell gene expression patterns. The lab has also used CellTags for tagging two cell populations...libraries at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...

  2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...single-molecule super resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent...fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks (max excitation...

  6. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg. Plasmid Serotype Name...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...overlap extension PCR. Although each type of cloning has its advantages, most scientists still encounter several...science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning...

  11. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...Avengers This year Addgene, where my wife Lianna has made her career as Senior Director of Biology, is...pre-clinical development for First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ... power of science to improve the world around us has long been known; ensuring all have access to this...information becomes easier to access by the day, it has never been more crucial to understand just how we...

  13. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...the field and terminology that is widely used but has a very specific use-case (definition) in a specific...specific field.  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the ...

  14. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright red pigment... University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful imaging.   ...

  15. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...available, the next best thing is a protein that has been validated for an assay that uses similar sample...Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western because...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...protein that no other protein in the expression system has. This is where affinity tags come in – they are peptides...solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...

  17. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...scheme, known as the Baltimore replication classes, has been developed around how they replicate (Figure ..., which retains the protein coat (and envelope), has a “gutted” genome with genes central to the replication...

  18. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...sciences research. The rare disease research community has certainly noticed: This year, the BeHEARD (Helping...McSweeney (right), a graduate student at Duke who has recently moved to Columbia University with David ...

  19. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ...

  20. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...enables you to discuss a person's performance or what has been learned, it helps to plan effective next steps...about areas they need to improve in. If a manager has concerns about an employee and the employee doesn...
Showing: 321 - 340 of 727 results