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We narrowed to 878 results for: IND

Showing: 321 - 340 of 878 results

  1. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... are interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide... and recombination-mediated genetic engineering (find more information on bacterial engineering using ...Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the ...expressing fluorescent proteins will be used to tag individual strains of bacteria. Their interactions and growth...University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria...PhD Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University of Ibadan. ...regulation and diabetes will be expressed from plasmids (find more information on bacterial expression systems...

  2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...found yourself frustrated reading through a paper to find that there is insufficient information about which...also designed to be persistent and exist online indefinitely. PIDs historically referred to objects that ...identify a plasmid available through Addgene and find verified information about each plasmid item. Given...associated plasmid page on the Addgene website and find specific information about the plasmid: name, description...and more. The plasmid page also helps researchers find other publications that have used the same plasmid... Addgene ID # and the information necessary to indicate that this ID is managed by Addgene. How to cite...down to the bottom of this plasmid’s page you will find that the “How to cite this plasmid” section now ...

  3. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such... left the arduous task of virus production to individual labs, but now we’re very excited to provide ready-to-use...an entire lentiviral production protocol only to find that the low titer of her prep makes it unuseable...target these widely studied signalling pathways. Find Validated gRNAs to use with your Cas9 Virus Pooled...uses nearly 80,000 gRNAs to target around 20,000 individual genes. We provide enough lentivirus for you to... lentivirus production protocol here and you can find more tips and protocols for working with lentivirus...Viral Service Page Check Out Our CRISPR Guide Pages Find Viral Vectors ...

  4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...size of the insert. You can search NCBI for YGOI to find the particular reference sequence if necessary. ...The plasmid was digested with 2 unique enzymes (HindIII and BamHI) and run on an agarose gel. The resulting... (*). When the plasmid is digested with either HindIII and BamHI alone (lanes 4-5), there is a single ...size of the plasmid. The double digest with both HindIII and BamHI (lane 3) produces bands at 6kb and 1.2kb...bromide (EtBr) to your gel before pouring it. EtBr binds to the DNA and allows you to visualize the DNA under...vist Addgene's resource for plasmid verification to find additional tips and detailed protocols on topics...Resources: Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find resources and software for your...contains Cas9. The Madison lab also created an inducible SRIRACCHA system (iSRIRACCHA) where Cas9 expression...a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al...leaving you with just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved...create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall ...Addgene blog Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher ...

  6. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...plasmids to help academic and industrial labs accelerate research and to help find a faster solution to the...methods Resources on Addgene.org Find all plasmids from Ginkgo Bioworks  Find COVID-19 plasmids for your research...current global pandemic in which scientists race to find vaccines, therapeutics, and other treatments to ...continually added as they complete QC and you can find all available plasmids listed on Ginkgo’s collection...global pandemic. And the reach of these plasmids is indeed global. Helping scientists speed up research is...

  7. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...Resources on Addgene.org Find base editors for your research Read our CRISPR guide Find plasmids for your research...modifying enzyme to the target site, and the deaminase induces the DNA base change without a DNA double-strand...for base editors is that the editing domain must induce the base change before the Cas domain lets go of...Cas9 domain to “hold on” long enough to the DNA to induce a base change. Because of this, ABEs are only weakly... DNA and off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and ...adenine base editors can correct all four of these kinds of point mutations, in theory converting the disease-associated...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...novice and indicate why other neuroscience newbies might want to get into the field. Find Optogenetics...neuroscience but are afraid you don’t have the right kind of biological expertise to get into the field, there...require a bit of expertise and can be difficult for individual labs to make efficiently. For select viral vectors... to get some ideas for future experiments and to find tools that will make your transition into the field... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request...Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ...

  9. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational approaches...Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find Our List of CRISPR gRNA Design...data sets (myself included) and we are faced with finding the best system to document these types of analyses...bioinformatics. In fact, there are endless ways and everyone finds their own. Here, I simply outline what works for...not apply or may not work for you. The best way to find a system that does is through trial and error, and...

  10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...mentors as one key to their success. But how do you find these elusive teachers, supporters and advisors?...trainees more about peer mentoring. They want to find mentors to help them develop their skills and to...accountable for their development plans. However, they find it awkward to ask someone for the time commitment...mentors available to go around. My advice has been to find 5 interested peers and form your own peer mentoring... steps to forming your own peer mentoring group Find 5-8 colleagues that you think might be interested...diversity of perspective. Sometimes it is helpful to find peers that share a similar direction or problem....potluck dinner. Invite guests from a lab, career or industry of interest – learn about their career paths and...

  11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... Resources on Addgene.org Find ready to use AAV vectors at Addgene. Find AAV plasmids to use in your... other. When Cre is introduced to the system it binds one pair of lox sites, let's say the loxp pair, ...expression?   Figure 1.  Cre-recombinase binds to either pair of lox sites, let's say the lox2272... the level of spontaneous recombination. This indicates that the amount of leaky expression can largely...recombination levels as high as 1-3%. In light of this finding, we now include guidance on the plasmid page of...levels of TH mRNA without expressing TH protein indicating that there is promoter activity. Additionally...Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https://doi.org/...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...typical part-time or 9-to-5 job. I had to ask to find out what a “postdoc” is, and that “PI” stood for...experimental design, and execution. Can lead to an independent project. Lab technician / Research assistant...next career stage (ex: grad school, med school, industry) Other roles you might see Lab manager: ...be unsure, it helps to have a vague sense of what kind of research you would be excited about. I know quite... in high school, but I’ve learned the value in finding mentors wherever I go. This story by Dr. Erika ... to Become a Scientist While Poor” Keep an open mind as you explore opportunities The world of science...doing scientific research. It’s okay to change your mind about what exactly you want to do—I started college...

  13. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ... Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization...are visible. These whorls can be quantified for individual cells, and the number of 'normal' looking cells...the results of the OSER assay, you should keep in mind that the presence of whorls is probably concentration...monomeric behaviour in cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers...is detected by Constantini et al (2015) and by Bindels et al (2017) but not by Cranfill et al (2016))....build a valuable resource with tools and data that indicate the performance of (a set of) fluorescent proteins...

  14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...days prior to the implementation date, our data indicated that, while 69% of all postdocs were expected ...stakeholders. However, while most institutions are indeed going ahead with their planned raises, some are...had that increase cancelled. Rounding out the remainder, 2% of postdocs who weren’t going to get a salary... reversed their position and decided to allow individual departments to go ahead with the raises as they...detrimental effect on the morale of postdocs, and indeed we have been contacted by a number of postdocs ...to reach out to institutions with a statement indicating that we encourage them to keep raising salaries...Variety of Science Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...

  15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering... Wong. The complexity of biological systems can hinder our attempts to study and engineer them, but what...Gilbert et al., 2014; Konermann et al., 2014) individual genes for studies interrogating their functions...incredibly useful for the efficient generation of individual (and multiple) genetic perturbations in single...sequences via oligo synthesis using the format indicated below and pool-clone them into the pAWp28 storage...identifies genes essential for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683....

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ... alternative evidence and discard inconvenient findings. Yet, we cannot prosper in a world where our medicines...ominous political climate in which American scientists find themselves today, and where Canadian scientists ...the public conversation and to tell the stories behind our research.  That's why I marched for science...University of Michigan campus speakers thoughtfully reminded us to use the same critical lens on our scientific... Aguilar Galileo wrote, “now you would not hide behind these two fingers the ninety-nine cubits of Aristotle...promote harmful policies that benefit polluting industries.In March, three climate scientists and an economist...specific, technical manner, so much so that we often find it difficult to convey the excitement and importance...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check..., 2018). For example, AcrIIA4 mentioned above indicates that it’s the fourth Acr protein discovered that...CRISPR activity one of three ways: Inhibit DNA binding Example:  AcrIIA4 blocks Cas9’s interaction with...2019). Inhibiting DNA cleavage Example: AcrIIC1 binds to the HNH endonuclease domain of Cas9 and prevents...such as inhibiting crRNA loading, inhibiting DNA binding, or inhibiting DNA cleavage.   Five ways to ...of CRISPR activity Regulating Acr proteins by inducible promoters, light, or small molecules could achieve...

  18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...interest  Figure 1. When ABEs are in action they bind DNA so that the TadA domain can edit the short single-stranded...creation of accidental insertions and deletions (indel) by the catalytically impaired D10A nickase from... efficiency in a human cell line while reducing indel frequency more than 90% compared to ABE7.10. Choosing...changes in cellular DNA and RNA dependent or independent of the gRNA used (Gaudelli et al., 2017). The...in this work did not cause genome-wide, guide-independent off-target deamination, an important attribute...0491-6 Additional resources on the Addgene blog Find more blog posts about base editing Learn about another...Addgene.org Get the basics of CRISPR in our CRISPR guide Find all plasmids for base editing in our collection ...

  19. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all...do this, the group developed a new strategy for finding retinal cell-type specific promoters. Most existing... mouse cell type specific transcription factor binding sites interspaced with random sequences.   Strategy...transcription start site. TFBS: transcription factor binding site. Image from Jüttner et al., 2019 with permission...that was active in the outer part of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic...Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting using AAV ...

  20. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...dividing cells. Find tips on using gamma-retroviral vectors in our Gamma-retrovirus guide and find popular packaging...tetracycline inducible, 3rd generation lentiviral vector backbone from the Shih lab. You can find more lentiviral... vectors. These vectors can induce combinatorial constitutive or inducible gene overexpression, knockdown...-lox system is a technology that can be used to induce site-specific recombination events and is widely...
Showing: 321 - 340 of 878 results