We narrowed to 798 results for: KIN

...with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids...of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were...CRISPR editing. Conclusions & implications One striking feature of this paper is the failure of the embryos...comprised of individuals with varied backgrounds. Talking about this study with non-biologist friends Like...

...the design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences...their recognition sequence, creating four base flanking overhangs. Since these overhangs are not part ...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...

... one place... well, lucky for you one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and...for low-cost, high-quality antibodies for IHC. Working with collaborators and support from the Brain Research...enable scientists to have an intuitive guide for checking their staining and neuroanatomy without the fear...by submitting data, sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman...

...localization in cells, or even single-particle tracking of the bound complex. There are so many FPs available...TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells... can even be bright enough for single-molecule tracking in live cells.  Controls  When designing experiments...Proteins Plasmids and Resources Reference Feng, S., Sekine, S., Pessino, V. et al. (2017) Improved split fluorescent...

...  Second, plasmids are easy to store and share, making recombinant antibodies the most practical choice... and producing antibodies from plasmids is much kinder to our animal friends! Finally, if we want better...improve genetically-encoded research tools, it is shocking that the plasmids and sequences for antibodies...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...Epigenetic engineering with single-pot assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms... own engineering plasmid tools in about a week. Taking advantage of Golden Gate cloning, the Haynes lab...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....

...tweet in order to easily access it later. Pro tip Checking the number of retweets, likes, and comments on...recent papers that the scientific community is talking about and jumping into the discussion! Semantic...altmetric score, is also displayed in your feed. By clicking on a paper in your feed you can see suggested ...Career Research Toolbox blog posts on free tools for making scientific graphics post and social media for scientists...

...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...reagents and strategies are not available to those working with yeast or plants. Using MoClo, or better yet...

...by starting with the least-abundant protein and working toward the most abundant. If the abundance of your...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing...ll want to check for the primary antibody by re-blocking your membrane and re-incubating with your secondary...is the one time in western blotting that you’re looking for a “blank” result! If some signal remains, you...

...This disease is caused by mutations in the common cytokine receptor gamma chain (c) and is characterized ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...AAV-based gene therapies are promising, scientists are working to overcome key challenges to their efficacy:  ...AAV vectors used to treat them. Scientists are working to overcome this issue by developing new capsids...

...Hi-C (Han et al., 2018). Think of 3C methods as taking a snapshot of chromatin looping with the photo ...CAPTURE method is similar to other 3C methods: crosslinking captures chromatin interactions, a restriction...together to create loops of DNA. This is key to linking pieces of DNA that are far away from each other...Zhang, Y., Liu, Y., Liu, N., Botten, G.A., Cao, H., Orkin, S.H., Zhang, M.Q., & Xu, J. (2020). Multiplexed...

...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful... see if your cloning experiment worked just by looking at the cells on the plate. For example, if your... identification is a good place to start before taking on more time consuming and expensive methods. While...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

...approach called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics (STRACK) to study stem ...While many barcoding libraries are available for tracking cell lineage, different applications require careful... can transduce neurons for retrograde labeling, making it a powerful tool for circuit tracing and other...

...Whether you’re new to in-house virus production or looking to improve your current methods, understanding ... add an additional viral passage. This involves taking your first round of virus, called P1 (or sometimes... and may need to be optimized for your purpose. Making your decision The final decision of which system...10430340260355347. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1977). The establishment...

...Terskikh et al.) and even displays predictable kinetics over 14 hours of expression in C. elegans embryos...Miyatsuka et al.) Distinguish between models for trafficking to the lysosome by fusing Medium-FT to LAMP-2A...structure and a particular biological event (trafficking to a subcellular location, start of gene expression...change color from blue to red report on cellular trafficking." Nature chemical biology 5.2 (2009): 118-126...

...the development of a simple and scalable means of making targeted genetic edits. Cas9 is a DNA binding protein...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can be reprogrammed...potent Cas9-based transcriptional regulator without making any functional changes to Cas9. For instance, when...PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer modeling." Cell...

...enter industry since I was drawn to the idea of working as a team to develop therapies that could directly... since you can’t afford to spend too much time working on something extremely risky or exploratory. Getting...it does not divulge any propriety information. Working as a team: Industry is all about teamwork. A single...environment, surrounded by lab members who are all working on related topics, scientific discussion is often...

...editing!  CRISPR functions in use But exactly what kind of editing are people doing with all these CRISPR... modifier. We used these categories to see what kinds of CRISPR tools people were depositing and requesting... share useful tools, and as such, we’re always looking to understand our community better. We therefore...year, or approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms...

... unknown, it is entirely possible that you are working with a low copy plasmid. When increasing the culture..., or a home-brew optimized for plasmid yields. Spiking your media with supplements such as magnesium salts...can optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn...

...Institute and Harvard Medical School. Scientists making transgenic organisms with Cas9 should be aware ...should be conducted with extreme caution. Why does making transgenic organisms with Cas9 risk creating a ...and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create an RNA-guided...mosquito species. Reproductive confinement involves working with laboratory organisms that can't reproduce ...