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Showing: 321 - 340 of 398 results

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Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

Published

April 6, 2021, 12:30 p.m.

...library in question. We will order primers that can amplify the insert of the library. This PCR step also adds...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

Published

Oct. 6, 2020, 1:15 p.m.

... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....
Mouse Modeling, Part 2: Breeding and Crossing Mice

Type

Blog Post

Published

Aug. 6, 2019, 12:55 p.m.

...reproduction include embryonic lethality, a short lifespan, or infertility and can arise due to copy number...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

Published

July 18, 2019, 12:55 p.m.

... resources and tools. That message was fully exemplified in Cori Bargmann’s keynote speech and in the ...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

Published

March 15, 2016, 2:30 p.m.

...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...
Gaining Leadership Skills Volunteering at a Professional Organization

Type

Blog Post

Published

Aug. 6, 2015, 2:30 p.m.

... skills can only assist you and make you more qualified for future career opportunities. Becoming involved...
Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

Published

July 23, 2015, 2:30 p.m.

...ask your local makerspace if they want to hack new life into it! If you’re in the Victoria area, please ...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

Published

Feb. 4, 2020, 2:15 p.m.

... lab developed as an acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy...
Antibodies 101: Conventional vs Spectral Flow Cytometry

Type

Blog Post

Published

Aug. 3, 2023, 1:15 p.m.

...Clinic studying host-microbe interactions in early life. Resources More resources on the Addgene blog...
Transferable Skills Guide: Managing a Team

Type

Blog Post

Published

Jan. 30, 2018, 2:42 p.m.

...undoubtedly negatively impact your work and your life. It’s up to you to decide which one you want to...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

Published

May 4, 2017, 2:30 p.m.

...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

Published

Jan. 10, 2017, 3:37 p.m.

...Lance-Byrne is a graduate student at the University of California, Santa Cruz, where her research is focused on...
Tips to Make the Most of a Scientific Conference

Type

Blog Post

Published

May 23, 2019, 12:26 p.m.

...you’ve interacted with on science Twitter in real life. If there is not a tweep up, create one yourself...
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

Published

April 4, 2019, 12:53 p.m.

...and Pamela A. Silver. "Harnessing undomesticated life." Nature microbiology 4.2 (2019): 212. PubMed PMID...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

Published

Oct. 23, 2018, 11:49 a.m.

...critical to the experiment, they definitely made my life easier! In this post, I’ll share some AAV production...
Hot Plasmids: Winter 2025

Type

Blog Post

Published

March 11, 2025, 1:15 p.m.

... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment. Figure...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

Published

July 13, 2017, 2:30 p.m.

...bees when it comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos and...
Transferable Skills: Negotiation

Type

Blog Post

Published

March 3, 2022, 2 p.m.

...a manuscript? And that’s only in your scientific life! I’m certain that you also negotiate with friends...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

Published

June 12, 2014, 3:30 p.m.

...I chose the 96 most useful things for day-to-day life in a plant lab, so it includes everyone’s favorite...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

Published

July 30, 2020, 1:15 p.m.

...future rabbit holes. For the non-scientists in your life, the book may be the introduction they need to grasp...

Showing: 321 - 340 of 398 results

Results per page:

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Mouse Modeling, Part 2: Breeding and Crossing Mice

Open Resources and Plasmid Tools For Studying C. elegans

Tips for Titering Your Lentiviral Preps

Gaining Leadership Skills Volunteering at a Professional Organization

Experimenting in an Open Source lab: from CRISPR to Cats

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Antibodies 101: Conventional vs Spectral Flow Cytometry

Transferable Skills Guide: Managing a Team

Fluorescent Proteins 101: Fluorescent Protein Timers

Truncated gRNAs for Regulating Gene Expression

Tips to Make the Most of a Scientific Conference

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Tips for a 1st time AAV user (by a Rookie AAV user)

Hot Plasmids: Winter 2025

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Transferable Skills: Negotiation

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Capturing a Moment with Marc Zimmer’s “The State of Science”

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