We narrowed to 405 results for: Lif;

1. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...reproduction include embryonic lethality, a short lifespan, or infertility and can arise due to copy number...

2. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ... resources and tools. That message was fully exemplified in Cori Bargmann’s keynote speech and in the ...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

4. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   .... In short, the Fragmid system takes the heavy lifting out of CRISPR vector design, making it easier to...

5. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...If you are lucky in lab life, you will have a plethora of antibody options for your experiment (all well...

6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...fluorescent proteins for protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One....

7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...

8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...library in question. We will order primers that can amplify the insert of the library. This PCR step also adds...

9. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...

10. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ... skills can only assist you and make you more qualified for future career opportunities. Becoming involved...

11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...ask your local makerspace if they want to hack new life into it! If you’re in the Victoria area, please ...

12. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...you’ve interacted with on science Twitter in real life. If there is not a tweep up, create one yourself...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...and Pamela A. Silver. "Harnessing undomesticated life." Nature microbiology 4.2 (2019): 212. PubMed PMID...

14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...critical to the experiment, they definitely made my life easier!  In this post, I’ll share some AAV production...

15. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...undoubtedly negatively impact your work and your life. It’s up to you to decide which one you want to...

16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...

17. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...Lance-Byrne is a graduate student at the University of California, Santa Cruz, where her research is focused on...

18. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...bees when it comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos and...

19. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ...Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification...

20. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...