We narrowed to 676 results for: Mos;
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TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/Cas moieties carry out these...Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org/wiki/File:Crispr.png ...
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A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....widely used in synthetic biology, biotechnology, and almost every microbiology lab,” says Di Ventura. It relies...still reached the level of expression needed for most applications. Figure 1: With blue light... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation maxima...EGFP does. Find mWasabi plasmids here! One of the most notable things about these proteins is that they... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m.... on Twitter if you missed our live updates! My most popular tweet from the conference is shown above...nucleases, but with a session on DNA repair. What stuck most in my mind from that session were words from Eric... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....interface with every possible option, we place the most important controls front and center, and make specialized...features. This algorithm enables one of SnapGene’s most popular aspects - its ability to annotate a raw ... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m.... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...that create a toxic product. The E. coli strain Most E. coli strains can be used to propagate plasmids... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....that they then tuck the science into. There are almost limitless storytelling options, and they all can...these storytelling devices, we sometimes forget the most important thing about science: It’s cool. Like bowties... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m.... on the Leith river. Photo courtesy of Leonardo Ramos. I enrolled into a summer school course at ...confluence of science and the world it hopes to change. Most researchers are aware of the importance of science... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....Breakdown of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids...Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career Posts... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....introduce a gene of interest into primary cells, so most primary cell lines require viral infection. A new...a new idea, but it comes with a few caveats. The most common protein-only transduction strategy is to ... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....Hernández Sánchez from Crowdfight. In March 2020, while most of the world was locked down, a South African company...competitiveness of cutting-edge research, is still one of the most valuable features of modern science in our view.... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....bench scientist who began working remotely recently? Most of us at Addgene began working from home last week... negative data or publish brief, novel findings. Most Micropublications contain one figure. If you aren... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....PMCID: PMC3574576. 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila... for transgene expression, lineage tracing, and mosaic analysis., Cell 141, 536–48 (2010). PubMed PMID... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....had to think creatively so as not to develop the most obvious strategy which also could have been proposed... In the end, the students had fun while applying most of the concepts required by the course and some ... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....Sweet, Vice President of Editorial at Cell Press. Almost everyone who works in a lab struggles with reproducibility...research environment. Journals as enforcers Currently, most of the burden for pushing for more transparency ... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....s writing will capture the attention of even the most skeptical reader of historical fiction (I can personally...’s The True Deceiver. There have been books that most of the group really disliked, some that everyone... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....Yamanaka himself, during his keynote address, appeared almost incredulous that the field has exploded so quickly...applications with the potential to save lives. While most scientists recognize and appreciate the importance... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....Postdoctoral Research Fellow with Pamela Silver. Most types of biological motion (whether endocytosis,...cartoony explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis,... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....meeting with no idea why you are there. Make the most of everyone’s time by considering the level of meeting...to meet monthly, but we found our meetings took almost two hours and we felt out of touch. By moving to... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....Updated Mar 26, 2020. At their most basic level, CRISPR/Cas9 genome editing systems use a non-specific...crRNAs from pre-crRNAs derived from CRISPR arrays. In most systems used for CRISPR-mediated genome editing,...