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We narrowed to 691 results for: Mos;

Showing: 321 - 340 of 691 results
  1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...communicate exactly what a site offers to their users, and most importantly, allow those users the ability to quickly...upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile, but occasionally you might...
  2. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... of information to fit into a single plasmid, so most first-generation binary vectors are quite large ...promoter. Originally isolated from the Mirabilis mosaic virus, the constitutively active M24 promoter has...
  3. Are Hybrid Guide RNAs Right for Your CRISPR Application?

    Type
    Blog Post
    Published
    Jan. 27, 2026, 2:14 p.m.
    ...tail sequence is much more forgiving and can be mostly or entirely replaced with DNA bases while retaining...required for best results. Different targets benefit most from different DNA substitutions, although there...
  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...they are peptides which do not naturally exist in most expression systems that can be added to the N or...downstream applications Below, we describe a few of the most common tags available. Maltose binding protein Maltose...
  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...Montgomery, or Louis Miller led these sessions. The most beneficial aspect of this course was breaking up...works.  4. Practice writing everyday Overall, the most important instruction the classes taught me was ...
  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...display protein aggregation, it’s recommended for most imaging applications. What can you do with SunTag... experiments in their paper, and so can you! In almost any case you would use a traditional FP fusion,...
  7. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics... be very informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to ...
  8. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...advancing the field.   What is a plasmid? At their most basic level, plasmids are small circular pieces ...that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria, but also...
  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...levels of an organization and is also one of the most common criteria required for a promotion. Yet little...fulfilling the requirements of the current task. Most of us start as doers, some become managers and some...
  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/Cas moieties carry out these...Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org/wiki/File:Crispr.png ...
  11. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ... on Twitter if you missed our live updates! My most popular tweet from the conference is shown above...nucleases, but with a session on DNA repair. What stuck most in my mind from that session were words from Eric...
  12. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...interface with every possible option, we place the most important controls front and center, and make specialized...features. This algorithm enables one of SnapGene’s most popular aspects - its ability to annotate a raw ...
  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Breakdown of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids...Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career Posts...
  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...that they then tuck the science into. There are almost limitless storytelling options, and they all can...these storytelling devices, we sometimes forget the most important thing about science: It’s cool. Like bowties...
  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ... on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into a summer school course at ...confluence of science and the world it hopes to change. Most researchers are aware of the importance of science...
  16. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...that create a toxic product. The E. coli strain Most E. coli strains can be used to propagate plasmids...
  17. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...introduce a gene of interest into primary cells, so most primary cell lines require viral infection. A new...a new idea, but it comes with a few caveats. The most common protein-only transduction strategy is to ...
Showing: 321 - 340 of 691 results