We narrowed to 342 results for: SEF;
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TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....proceeds in an informed fashion. For science to be useful to governments, however, inconvenient data must...
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Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....near-infrared) (Qian et al., 2019). NIR FPs are useful in vivo probes, due to low autofluorescence, low... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....played by microtubules, Opto-katanin will surely be useful for a wide variety of experiments. Opto-RhoGEFs... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....their solubility, and thus different reagents are useful for biotinylation in different microenvironments... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....plasmids described above, Addgene carries several useful CRISPR toolkits for creating plant expression plasmids... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....largely replaced these, these techniques still have useful applications and have informed many current techniques... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....the other long-homology based cloning methods are useful alternatives to the standard restriction/ligation... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....junction. In zebrafish, the method has become very useful in lineage tracing; “Zebrabow” was used to trace... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....able to apply someone else's new and incredibly useful technique simply by following the abbreviated methods... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....for every experiment, at least one will likely be useful. Optimal gRNA selection With some CRISPR experiments... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m.... concentration of recovered DNA as this will be useful for the ligation step. 3. Ligate Your Insert into... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....'t like speaking, they don't feel like they get useful feedback, and they don't feel like they've made... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data often ... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....through multiple focal planes of a sample may also be useful for providing fast imaging along with optical sectioning... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs. Table 2:... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m.... that can pass through cell membranes can prove useful for live-cell imaging. Experimental context and... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....be added one by one. This approach is especially useful for combinatorial analyses. For example, if you... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....alter the genome and chromatin architecture, it is useful to regulate the time in which dCas9 binding occurs... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m....Sanger sequencing). See Wang et al. (2013) for a useful reference. Q14: I have used 200ng to 500ng of ... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....continued replication of the virus. While this can be useful, if too many neurons are labeled, it can be difficult...