We narrowed to 499 results for: SHA
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TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m.... We use writing to communicate our findings and share them with the world. If one doesn’t write and publish...
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Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....and endonuclease domains, all four Rep proteins share a common helicase and ATPase domains. To function... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m....nickases improve homology directed repair rates, and share their design rules for your next CRISPR nickase ... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....science and supporting scientists globally are the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....three years ago I decided it was time to get in shape. After three kids, I had put on typical middle age... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....Successful People Become Even More Successful, by Marshall Goldsmith, with Mark Reiter Harvard Business... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....before in your field?” He points out to “not be shackled by your own subject - maybe something used in ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m....Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent cells to save... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....have good observations and be less inhibited in sharing concerns. Ask alumni of the lab. Most labs have... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....All organisms share an innate goal to survive. This past year, scientists hijacked survival tactics of... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....well, don’t relax just yet. Proteins often change shapes and epitope accessibility naturally, by binding... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....primary antibodies and the targets will be indistinguishable (top panel). If one of the antibodies is converted... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists would be able to ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....process isn’t new to Addgene, we are excited to share the procedures and Python scripts with laboratories... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....conversation — put your work on preprint servers, share your email address far and wide, keep your website... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....periodically for possible new awardees, and we’re happy to share stats and strategies with tech transfer offices.... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar gene expression... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....development. Today, ABRC does more than create and share resources, we seek out opportunities to engage people...