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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 634 results for: SON

Showing: 321 - 340 of 634 results

  1. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the...

  2. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular...

  3. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...gene and cell-based therapies in the new era of personalized medicine (2017).   1st Gen AdVs are stripped...gene and cell-based therapies in the new era of personalized medicine." Genes & diseases 4.2 (2017): 43-63...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body’... 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...and inhibitory synaptic proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal...library from Addgene New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...antibodies recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). ...

  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources References...Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation.      New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...20463753. PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in...

  12. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet searches are done via...potentially faulty product. Please remember that the person at the other end of that email you’re writing is...

  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...and so should be handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...panelist or audience member from taking over. My own personal rule is that I do not let the panelists introduce...stop talking and redirect the conversation. The person you cut off might be miffed but the other 200 audience...

  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ...

  18. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination...  Rachel Leeson...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR ...
Showing: 321 - 340 of 634 results